中文摘要：

目的探讨子宫内膜异位症（EMS）患者在位内膜腺上皮细胞中骨桥蛋白（OPN）对核因子κB（NF-κB）p65表达的影响及其与细胞侵袭的关系。方法原代分离培养12例EMS患者在位内膜原代腺上皮细胞。采用OPN siRNA干扰细胞24h后收集,Western blot、RT-PCR方法分别检测干预前、后细胞中OPN、NF-κBp65蛋白及其mRNA表达情况;Transwell试验检测干预前、后细胞侵袭性的变化。采用相同剂量的无RNA酶水干预EMS患者在位内膜腺上皮细胞作为未干预组。结果与干预前比较,OPN siRNA干扰后细胞中OPN蛋白、mRNA的表达明显下降（t1=7.92,t2=9.87,P〈0.05）;与未干预组比较差异无统计学意义（P〉0.05）;OPN siRNA干扰后细胞中NF-κBp65蛋白及mRNA的表达明显减弱（t1=-2.13,t2=-8.61,P〈0.05）,与未干预组比较差异无统计学意义（P〉0.05）。OPN siRNA干扰后原代腺上皮细胞的侵袭性明显降低（t=2.38,P〈0.05）,与未干预组比较差异无统计学意义（P〉0.05）。OPN、NF-κBp65在EMS患者在位内膜腺上皮细胞中的表达呈明显的正相关（r=0.87）。结论 OPN siRNA沉默EMS患者腺上皮细胞中的OPN后OPN、NF-κBp65的表达及细胞的侵袭性均明显降低,且二者呈明显正相关,揭示二者极有可能在异位细胞的侵袭中发挥同步协调作用。

英文摘要：

Objective To explore the influence of OPN in eutopic glandular epithelial cells of endometriosis on the NF-κBp65expression and its relationship with the cell invasion.Methods The eutopic primary glandular epithelial cells in 12 cases of endometriosis were performed the primary isolation and culture.The cells were collected after 24 hOPN siRNA intervention.Western blot and RT-PCR methods were adopted to detect the expressions of OPN,NF-κBp65protein and its mRNA before and after intervention.The Transwell experiment was used to detect the change of cell invasiveness before and after intervention.Results The expression of OPN protein and mRNA after interfering primary glandular epithelial cells by OPN siRNA was significantly decreased,and the difference was statistically significant（t1=7.92,t2=9.87,P〈0.05）.the expression of NF-κB p65 protein and mRNA after OPN siRNA interfering primary glandular epithelial cells was obviously weakened,the difference was statistical significant（t=2.38,P〈0.05）.the invasiveness of primary glandular epithelial cells after OPN siRNA intervention was significantly decreased,the difference was statistically significant（t=2.38,P〈0.05）.The expression of OPN and NF-κBp65had a significantly positive correlation in eutopic endometrial glandular epithelial cells（r=0.87）.Conclusion The expression of OPN and NF-κBp65is significantly decreased after OPN siRNA interfering eutopic endometrial glandular epithelial cells,therefore OPN most likely lead to the occurrence and development of endometriosis via the NF-κB pathway.