为探讨植物-微生物联合修复佳乐麝香(HHCB)与镉(Cd)复合污染土壤技术的可行性及其相关修复土壤微生物群落多样性的变化,采用紫茉莉(Mirabilis jalapa L.)与孔雀草(Tagetes patula L.)两种植物配合HHCB降解菌,对HHCB,Cd 复合污染土壤进行联合修复研究,并使用变性梯度凝胶电泳技术(DGGE)在微观层面上进一步分析土壤微生物群落多样性的变化趋势.结果表明: 孔雀草对Cd 的富集效果要好于紫茉莉,且两种植物地上部分对于Cd 的富集效果更好.HHCB 含量的增加会导致降解菌的降解效率降低,在HHCB含量为100mg.kg-1时,最好的降解效果可达到63.58%,在HHCB含量为300mg.kg-1时,最好的降解效果达到55.3%.土壤中HHCB 的含量升高,其细菌种群多样性指数呈现降低的趋势,添加Cd 也会使其细菌种群多样性指数呈现降低的趋势,HHCB 与Cd 复合污染的细菌种群多样性指数低于HHCB 与Cd 单一污染的细菌种群多样性指数.研究结果为HHCB 与Cd复合污染土壤的植物一微生物联合修复提供了一条比较可行的方法并为进一步研究其微生物多样性变化提供了一定的理论基础.
In order to explore the feasibility of bioremediation technology for contaminated soil by galaxolide (HHCB) andcadmium (Cd) with the plant-microbial combination, the jasmine (Mirabilis jalapa L.) and maidenhair (Tagetes patula L.), as wellas the galaxolide (HHCB) degrading bacteria had been used to remediate HHCB and Cd contaminated soil, and the dynamicchanges of the soil microbial community diversities were determinted with denaturing gradient gel electrophoresis (DGGE)technique.The results showed that the enrichment effect of maidenhair on Cd was better than that of purple jasmine, the aboveground parts of the two plants had better effect on the enrichment of Cd. The increase of HHCB content has led to thedegradation efficiency of degrading bacteria. The best degradation effect was 63.58% when HHCB content was 100mg.kg-1, andthe best degradation effect was 55.3% when HHCB content was 300mg.kg-1. The bacterial population diversity index showed adecreasing trend when the content of HHCB increased in soil. There was a decreasing trend of the bacterial population diversityindex in Cd pollution. Bacterial population diversity index in HHCB and Cd combined pollution was lower than that of HHCBand Cd single pollution bacterial population diversity index, which provides a feasible method for the combined remediation tothe HHCB and Cd contaminated soil.