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一种制备蝙蝠染色体标本的改良方法
  • ISSN号：2095-8609
  • 期刊名称：《昆虫分类学报》
  • 时间：0
  • 分类：Q789[生物学—分子生物学]
  • 作者机构：[1]绵阳师范学院珍稀动物生态与系统进化研究所,四川绵阳621000
  • 相关基金：国家自然基金（30499341）,四川省教育厅青年基金（200613083）
作者： 石红艳[1], 唐桂容[1], 刘昊[1]
关键词： 蝙蝠, 骨髓细胞, 方法, 染色体, bats , marrow - cell , method , chromosome
中文摘要：

对骨髓细胞蒸汽固定法进行改进,得到一种制备蝙蝠染色体标本的简便方法。在实验条件较差,实验经费少的条件下应用该方法能够制备出质量较好的染色体标本,尤其是对于初学者,采用该方法制备蝙蝠染色体的成功率较高。在开展蝙蝠等小型哺乳动物细胞遗传学研究方面具有很好的推广应用前景。

英文摘要：

A simple and convenient method to prepare chromosomes of bats is presented. The method ensures good spreading of chromosomes within metaphases, high frequency of metaphases suitable for counting and karyotyping. Compared with other chromosome methods, this method has the following advantages. 1 ） It is technically simple. 2） It ensured high success - rate in several systems tested, especially for beginners. 3 ） It can help us carry out cytogenetic work in some poor conditions e. g. when centrifuge is not available.

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期刊信息
  • 《昆虫分类学报》
  • 中国科技核心期刊
  • 主管单位:教育部
  • 主办单位:西北农林科技大学;中国昆虫学会
  • 主编：袁锋
  • 地址：陕西省杨凌区西北农林科技大学昆虫博物馆
  • 邮编：712100
  • 邮箱：kunchongfl@nwsuaf.edu.cn
  • 电话：029-87092524 87092024
  • 国际标准刊号：ISSN：2095-8609
  • 国内统一刊号：ISSN：61-1495/Q
  • 邮发代号:
  • 获奖情况:
  • 1992、1996、2000年连续三年被编入《中文核心期刊...,1999年陕西省优秀期刊,中国期刊方阵“双效”期刊
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  • 被引量:1709