中文摘要：

目的：运用基因芯片高通量筛选技术深入研究消癌解毒方抗恶性肿瘤单一信号通路及多靶点联合治疗的作用机制,从分子、蛋白及基因水平深层次揭示其抗癌机制。方法：中药组及对照组分别以低、中、高剂量消癌解毒方中药及0.9%氯化钠溶液灌胃3d,制备成含药血清,经细胞培养,全基因组表达谱芯片实验和实时荧光定量PCR技术检测消癌解毒方及在此基础上加入激动剂脂多糖（LPS）及TLR4阻断剂（CD284）对人肝癌细胞SMMC-7721的TLRs/NF-κB信号转导通路关键基因表达的影响。结果：高浓度含药血清＋CD284组与空白血清＋CD284组比较,下调表达了TLRs/NF-κB信号通路TLR4,TRAF-6等表达水平。并运用实时定量PCR法验证TLR4等m RNA和基因表达水平。结论：消癌解毒方能够明显抑制人肝癌细胞SMMC-7721的增殖,其机制可能是含药血清与CD284能协同作用于人肝癌细胞SMMC-7721的TLRs/NF-KB信号转导通路,抑制核转录因子NF-κB的异常持续活化,促进肿瘤细胞凋亡。

英文摘要：

Objective： To deeply study the mechanism underlying Xiaoai Jiedu Formula inhibiting cancer from the views of single signaling pathway as well as multi-target pathway by means of the gene chip technology, and to explore its anti-cancer mechanism from different levels including the molecular, protein and gene. Methods： To prepare the different dosages of drug containing serum, the administration group was given the low, medium and high doses of Xiaoai Jiedu Formula by gavage for 3 days. Then, Xiaoai Jiedu Formula containing serum was used to culture the human hepatocarcinoma SMMC-7721. Effects of Xiaoai Jiedu Formula containing serum added agonist LPS and antagonist TLR4（CD284） on critical genes expression in TLRs/NF-κB signaling pathway of SMMC-7721 cells were detected by the gene chip technology and real-time quantitative PCR. Results： The administration of high concentration of Xiaoai Jiedu Formula containing serum added CD284 downregulated TLR4 and TRAF-6 genes in the TLRs/NF-κB signaling pathway when compared to the administration of blank serum added CD284. The change in the TLR4 gene was verified with real-time quantitative PCR method. Conclusion： Xiaoai Jiedu Formula could inhibit human hepatoma SMMC-7721 cells proliferation, whose mechanism may be related to the synergistic action of Xiaoai Jiedu Formula containing serum and CD284 on TLRs/NF-KB signaling pathway of the cells and the inhibitory effects on abnormal activation of NF-κB, resulting in promoting the tumor cells apoptosis.