中文摘要：

目的：在中医药理论指导下,深入研究中医药抗恶性肿瘤单一信号通路及多靶点联合治疗的机制。方法：中药组与对照组分别以低（3.78g/kg）、中（7.56g/kg）、高（15.12g/kg）剂量消癌解毒方中药及0.9%氯化钠溶液灌胃3d,制备含药血清,经细胞培养,透射电镜观察肿瘤细胞凋亡及坏死微观结构,Western Blot法检测消癌解毒方及在此基础上加入激动剂脂多糖（LPS）及TLR4阻断剂（CD284）对人肝癌细胞SMMC-7721的TLRs/NF-κB信号转导通路的影响。结果：高浓度含药血清加入CD284剂量组电镜下可见人肝癌细胞株SMMC-7721变性,水肿,胞质细胞器广泛空泡变,部分细胞碎裂,坏死,胞核溶解,胞膜破裂,细胞器外溢;Western Blot法检测TLR4等mRNA和蛋白表达水平最弱。结论：消癌解毒方能够明显抑制人肝癌细胞SMMC-7721的增殖,其机制可能是含药血清与CD284能协同作用于人肝癌细胞SMMC-7721的TLRs/NF-κB信号转导通路,抑制核转录因子NF-κB的异常持续活化,促进肿瘤细胞凋亡。

英文摘要：

Objective： To deeply study the mechanism of TCM resisting single signal pathway of malignancy,and of multi-target combined therapy of TCM,based on the theories of TCM.Methods： Traditional Chinese medicine group and control group were respectively given a gavage of the low（3.78g/kg）,medium（7.56g/kg） and high（15.12g/kg） Xiaoai Jiedu Fang.Chinese medicine and physiological saline 3 days to prepare a serum containing Not only with cell culture,study of tumor cell apoptosis,necrosis microstructure and flow cytometry（double straining） by STEM,and assay to Xiaoai Jiedu Fang in Western-blot,but on the basis of that,added agonist LPS and antagonist CD284 of TLR4 to conduct the effects on signal transduction pathways TLRs/NF-κB of human heptoma SMMC-7721 cells.Results： After adding antagonist CD284 of TLR4,with STEM,serum containing high concentrations could be found that of which human hepatoma＇s cell line SMMC-7721 becomes degeneration,edema,cytoplasmic organelles are extensively vacuolated,and some cells become fragmentation,necrosis,nuclei dissolve,membrane rupture,organelles are spilled out at the same time;After adding TLR4＇s antagonist CD284 flow cytometry（double staining） in dose group,serum containing high concentrations are detected in highest rate of early plus late apoptosis;after adding antagonist CD284 of TLR4 in dose,mRNA like TLR4 and protein express is weakest by Western-Blot assay.Conclusion： Xiaoai Jiedu Fang can inhibit the proliferation of human hepatoma cells SMMC-7721,that is because containing serum and antagonist CD284 of TLR4 can be synergy in signal transduction pathway TLRs/NF-KB of human hepatoma cells SMMC-7721 to inhibit the abnormal and continued activation of nuclear transcription factor NF-κB and promote the apoptosis of tumor cells.