中文摘要：

利用电子克隆技术获得大豆中的苹果酸脱氢酶基因cDNA序列,并对基因及其编码蛋白进行生物信息学分析和预测,为进一步克隆该基因、解决植物磷高效基因数量缺乏等问题提供依据。结果表明,大豆中的苹果酸脱氢酶基因（GmMDH1）cDNA序列长度为1 574 bp,开放阅读框1 230 bp,编码409个氨基酸残基;编码蛋白含有NAD bindingsite,Dimerization interface,Substrate binding site,MDH_glyoxysomal_mitochondrial结合位点和保守域,属于LDH_MDH_like Superfamily家族;亚细胞定位分析显示,编码蛋白位于植物细胞的叶绿体中。

英文摘要：

To obtain more plant high phosphorus-efficiency related genes,one plant high P-efficiency gene,named malate dehydrogenase gene（MDH）,was isolated from soybean by using the in silico cloning technique.And the protein of this gene was also predicted and studied through the bioinformatics analysis method.The results showed that the cDNA length of soybean malate dehydrogenase gene GmMDH1 was 1574 bp,which included a 1230 bp open reading frame （ORF） and encoded 409 amino acids residue.The encoding protein of GmMDH1 possessed NAD binding site,dimerization interface,substrate binding site,MDH_glyoxysomal_mitochondrial,LDH_MDH_like super-family domains and was predicted to locate in cell chloroplast.