中文摘要：

在克隆获得磷高效相关转录因子基因GmPTF1的基础上,构建基因的超表达载体pGN-GmPTF1和无标记（Marker-free）表达载体pX6-GmPTF1;利用农杆菌介导子叶节与花粉管通道转化技术,将表达载体pGN-GmPTF1和pX6-GmPTF1分别转入冀豆12、冀豆16和五星1号大豆品种。转化后的大豆植株经PCR检测,有11株转化植株可扩增出目的条带,其中5株转有pGN-GmPTF1载体和6株转有pX6-GmPTF1载体,表明GmPTF1转录因子基因已初步整合至大豆基因组中。

英文摘要：

The over-expression vector pGN-GmPTF1 and marker-free expression vector pX6-GmPTF1 were constructed in this study base on the cloning and identification of GmPTF1,a soybean high phosphorus-efficiency transcription factor gene,and the vectors were introduced into different soybean varieties（Ji-dou 12,Ji-dou 16,Wu-xing 1）by Agrobacterium-mediated cotyledonary-node and pollen tube pathway transformation methods.PCR analysis results of transformation plants showed that the GmPTF1 was successfully incorporated into the soybean genome.Total five PCR positive plants of vector pGN-GmPTF1 and six PCR positive plants of vector pX6-GmPTF1 were obtained from T0 generation,respectively.