中文摘要：

以MYB转录因子GmPHR1为目的基因,冀豆12、冀豆16和绥农14为转化受体,比较农杆菌介导大豆不同外植体的遗传转化技术,为大豆转基因育种提供技术支撑。结果表明,以茎尖转化系统的不定芽诱导率、植株再生率和转化效率最高,且对基因型的依赖性最小,但由于对抗生素的敏感性较差,因而存在一定程度的假阳性;其次,以胚尖转化系统的不定芽诱导率、植株再生率和转化效率较高,对基因型的依赖性较小,同时对抗生素的敏感性较强,因而是一种较为理想的转化系统。而子叶节、下胚轴转化系统则表现出不定芽诱导率、植株再生率和转化效率均较低,且存在较强的基因型依赖性等不足。同时,利用上述4种转化系统,获得了3个供试大豆品种的转基因T1植株。

英文摘要：

In this study,the MYB transcription factor GmPHR1 was transformed into three soybean genotypes by Agrobacterium-mediated different explants.The results showed that the shoot tip transformation system was with the highest shoot induction,shoot regeneration,transformation ratios and little genotype dependence.But it was the least sensitive to antibiotics,so there were some escapes in this system.The transformation system with embryonic tip was higher in shoot induction,shoot regeneration and transformation ratios.In addition,it was more sensitive than others to antibiotics,so embryonic tip transformation was a more ideal soybean genetic transformation system.The cotyledonary node transformation and the hypocotyl transformation systems were lower in shoot induction,shoot regeneration and transformation ratios and significant differences among genotypes existed.By using the above transformation systems T1transgenic lines of the three soybeans were obtained.