中文摘要：

目的研究当归补血总苷（TGDGBX）对转化生长因子β1（TGF-β1）诱导的人胚肺成纤维细胞（HLF-Ⅰ）转分化及胶原表达的调控作用。方法体外培养HLF-Ⅰ,根据不同的处理方法分为5组,A组：对照组;B组：加入TGF-β1（1ng/ml）;C组：加入TGF-β1（1 ng/ml）＋TGDGBX（0.03 mg/ml）;D组：加入TGF-β1（1 ng/ml）＋TGDGBX（0.09 mg/ml）;E组：加入TGF-β1（1 ng/ml）＋TGDGBX（0.27 mg/ml）。MMT法检测各组HLF-Ⅰ的增殖情况,羟脯氨酸消化法检测细胞上清液中羟脯氨酸（HYP）含量,ELISA法检测细胞上清液基质金属蛋白酶-9（MMP-9）的含量。半定量RT-PCR法检测Ⅰ型胶原（COL-Ⅰ）mRNA、Ⅲ胶原（COL-Ⅲ）mRNA、α-平滑肌肌动蛋白（α-SMA）mRNA以及MMP-9 mRNA的表达情况。结果与B组相比,C、D、E组明显抑制TGF-β1诱导的HLF-Ⅰ细胞增殖（P〈0.05）;降低细胞上清液中HYP的含量（P〈0.05）;减少HLF-Ⅰ的COL-ⅠmRNA、COL-ⅢmR-NA以及α-SMA mRNA的表达（P〈0.05）;增加细胞上清液中MMP-9的含量（P〈0.05）。D和E组与B组相比,显著提高HLF-Ⅰ的MMP-9 mRNA表达水平（P〈0.05,P〈0.01）。结论 TGDGBX可抑制TGF-β1诱导的HLF-Ⅰ转分化和胶原表达,其抑制胶原表达的作用可能是通过增加MMP-9的表达来实现的。

英文摘要：

Objective To investigate the mechanism of TGDGBX regulating expression of collagen in human fetal lung fibroblast（HLF-Ⅰ） triggered by transforming growth factor-β1（TGF-β1）.Methods Fibroblasts was cultured by α-MEM medium and was grouped according to treatment.Groups as follows,A： control;B： TGF-β1（1 ng/ml）;C：TGF-β1（1 ng/ml） ＋ TGDGBX（0.03 mg/ml）;D：TGF-β1（1 ng/ml） ＋TGDGBX（0.09 mg/ml）,E：TGF-β1（1 ng/ml）＋TGDGBX（0.27 mg/ml）.Proliferation of cells was measured by MTT assay.The changes of collagen-Ⅰ（COL-Ⅰ）,collagen-Ⅲ（COL-Ⅲ）,α-SMA and MMP-9 mRNA was detected by reverse transcription-polymerase chain reaction（RT-PCR） and the expression of MMP-9 in the supernatants were detected with ELISA.Results The cell multiplication of groupe TGDGBX was significantly higher than that in group TGF-β1（P0.05）;The collagen Ⅰ（COL-Ⅰ）,collagen Ⅲ（COL-Ⅲ） and α-SMA mRNA expression of fibroblasts in group TGDGBX were significantly lower than that in group TGF-β1（P0.05）,but the expression of MMP-9 mRNA in group TGDGBX was higher than that in group TGF-β1（P0.01）;TGDGBX could induce up-regulation of MMP-9 content of the supernatant.Conclusion TGDGBX can inhibite collagen expression by reducing HFL-I to MFB transdifferentiation and increase the expression of MMP-9 mRNA of HLF-Ⅰ.