中文摘要：

目的建立HPLC-ELSD测定当归补血总苷（黄芪、当归）中的黄芪甲苷和黄芪皂苷Ⅱ的方法。方法色谱柱Kro-mail C18（4.6 mm×250 mm,5μm）;流动相为乙腈-水（37.5∶62.5）;体积流量0.8 mL/min;ELSD参数：漂移管温度100℃;N2气流体积流量2.60 L/min。结果黄芪甲苷在0.85～6.76μg之间呈良好的线性关系（r=0.999 2）,平均回收率为98.8%,RSD为1.50%。黄芪皂苷Ⅱ在1.05～8.4μg之间呈良好的线性关系（r=0.999 4）,平均回收率为95.7%,RSD为2.70%。结论该法精密度、重复性良好,简便、快速、准确,适用于当归补血总苷中黄芪甲苷、黄芪皂苷Ⅱ的测定。

英文摘要：

AIM To establish simultaneous assay of the contents of astragaloside IV and astragaloside Ⅱ in total glucosides of Danggui Buxue Decoction（Astragali Radix,Angelicae sinensis Radix） by HPLC-ELSD.METHODS Decoction was measured by using Kromail C18 as chromatography column and acetontrile-water（37.5∶ 62.5）as mobile phase.The flow rate of mobile phase was 0.8 mL/min.The tube temperature of the detector was 100 ℃,and the flow rate of N2 was 2.60 L/min.RESULTS The measured value of astragaloside Ⅳ showed good linear correlation between 0.85 and 6.76 μg（r=0.999 2）,with average recovery of 98.8% and RSD 1.50%.The measured value of astragaloside Ⅱ indicated favorable linear correlation between 1.05 and 8.4 μg（r=0.999 4）,with average recovery of 95.7% and RSD 2.70%.CONCLUSION The method is accurate,simple,fast and feasible and can be applied as the assay of astragaloside IV and astragaloside Ⅱ in total glucosides of Danggui Buxue Decoction.