中文摘要：

以克伦特罗（CL）为竞争半抗原,人工合成的克伦特罗-卵清白蛋白（CL-OVA）为包被抗原,两者与一定量的SD大鼠抗CL血清反应,建立快速定量测定CL含量的间接竞争酶联免疫吸附检测（ciELISA）方法.结果表明,理想的包被抗原质量浓度为3μg/mL,抗CL血清稀释倍数为1∶1.6×10^5.绘制出标准曲线,线性检测范围为4.88～5 000ng/mL,线性回归方程为y=-0.295 2x＋1.160 6,r^2=0.990 2,加标平均回收率为95.45%.该方法敏感性高,特异性强,操作简便,对于研制瘦肉精残留检测试剂盒有重要意义.

英文摘要：

Clenbuterol （CL） acted as competitive hapten, the conjugate OVA - CL of ovalbumin and clenbuterol acted as coating antigen, competitive indirect ELISA（ciELISA） for clenbuterol have been developed based on Sprague - Dawley （SD） rat antiserum against CL. The obtained optimum conditions of the assay follow ： 3μg/mL of the plate - coating antigen CL - OVA in carbonate sodium buffer, 1： 1.6 × 10^5 of antiserum titer. The detection range of this assay method is broadly various from 4.88ng/mL to 5 000ng/mL, and an average recovery of 90.67% is obtained. This method shows high sensitivity, strong specificity.