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猪小肠粘膜上皮细胞的分离及体外原代培养模型的建立
  • ISSN号：1001-991X
  • 期刊名称：《饲料工业》
  • 时间：0
  • 分类：S828[农业科学—畜牧学;农业科学—畜牧兽医]
  • 作者机构：[1]河南省农业科学院农业部动物免疫学重点开放实验室,河南省郑州市农业路1号450002, [2]华南农业大学动物科学学院
  • 相关基金：国家重点基础研究发展计划项目（2004CB117501）、广东联合基金项目（No.u0731004）和博士点基金项目（20070564014）资助
作者： 职爱民[1], 左建军[2], 黄志毅[2], 邹仕庚[2], 冯定远[1]
关键词： 猪, 小肠粘膜细胞, 原代培养
中文摘要：

用机械刮取小肠粘膜加胶原酶Ⅱ消化的方法，采用新生未吮乳仔猪小肠组织，成功地分离了猪小肠粘膜上皮细胞（Intestinal epithelial cells，IEC）且在体外培养传代，并采用碱性磷酸酶和角蛋白免疫学试验鉴定，结果表明：体外培养的猪IEC在DMEM—F12培养基中，细胞在1～2d贴壁，5～6d形成细胞克隆，9～11d融合成片；碱性磷酸酶和生物素标记的细胞角蛋白抗体鉴定结果显示90％培养的细胞为阳性，分别从生化的角度证明和细胞生物学角度鉴定了所培养的细胞为IEC。

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期刊信息
  • 《饲料工业：英文版》
  • 主管单位:
  • 主办单位:
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  • 地址：沈阳市长江街126号甲名化之都4-1501
  • 邮编：110031
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  • 电话：024-86875000
  • 国际标准刊号：ISSN：1001-991X
  • 国内统一刊号：ISSN：21-1169/S
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  • 被引量:1