中文摘要：

与多样的生物功能，小 bioactive 肽接受了在动物生产作为生理地有益的物质增加注意。到小 bioactive 肽的宽申请的主要障碍是为大量生产的划算的方法的缺乏。在这研究，我们把 glycyl 夫酸安(Gly-Gln ) 用作一个测试用例为小 bioactive 肽开发一个新奇大量生产方法。编码 Gly-Gln 的 oligonucleotide 支持肽(Gpp ) 被设计并且综合。Gpp 为胃朊酶和 trypase 包括 3 Gly-Gln dipeptides 和 2 个酶的地点，允许在胃肠的道的 Gly-Gln 的直接消化和吸收。Gpp oligonucleotides 被连接产生包含 Gpp 的 12 个双人脚踏车拷贝的一个 oligomeric oligonucleotide 片断。这个 12Gpp 片断在 Escherichia coli 向量 pET32a 被克隆并且表示。由优化文化条件[0.1 mmol L ? 1 isopropyl- 尾 - D-thiogalactopyranoside (IPTG ) ， 50 渭 g mL ? 1 氨比西林(安培) ，为 12 h 的 30 掳 C ] ， thioredoxin 熔化肽到达了 40% 全部的细菌的蛋白质。在纯化以后，熔化蛋白质被喂到 Kunming 老鼠在老鼠免疫者功能上决定它的效果。结果显示出那类似于 Gly-Gln dipeptide， Gpp 聚合物蛋白质能显著地在血和怒气压制 T 和 B 淋巴细胞的增长，并且能显著地另外改进 interleukin-2 (IL-2 ) 和 interleukin-6 (IL-6 ) 血和怒气的分泌物淋巴细胞。这些效果没在喂的老鼠被观察 2 氨基酸混合(glycine 和夫酸安) 。当摄取了进动物内脏时，这些证据显示 Gpp 聚合物蛋白质的有效消化能被完成。在这研究的表达式系统为不仅 Gly-Gln dipeptide 而且另外的短 bioactive 肽提供一个潜在的生产方法。

英文摘要：

Small bioactive peptides, with diverse biological functions, have received increasing attention as physiologically beneficial substances in animal production. The main obstacle to wide application of small bioactive peptides is a lack of costeffective methods for mass production. In this study, we mass-production method for small bioactive peptides. used glycyl-glutamine （Gly-Gln） as a test case to develop a novel The oligonucleotide encoding Gly-Gln pro-peptide （Gpp） was designed and synthesized. Gpp includes 3 Gly-Gln dipeptides and 2 enzymatic sites for pepsin and trypase, allowing direct digestion and absorption of Gly-Gln in the gastrointestinal tract. The Gpp oligonucleotides were linked to generate an oligomeric oligonucleotide segment containing 12 tandem copies of Gpp. This 12Gpp segment was cloned and expressed in Escherichia coli vector pET32a. By optimizing culture conditions [0.1 mmol L^-1 isopropyl-β-D- thiogalactopyranoside （IPTG）, 50 μg mL^-1 ampicillin （Amp）, 30℃ for 12 h], the thioredoxin fusion peptides reached 40% of total bacterial protein. After purification, the fusion protein was fed to Kunming mice to determine its effect on mouse immune function. The results showed that similar to Gly-Gln dipeptide, Gpp polymer protein could significantly suppress the proliferation of T and B lymphocytes in blood and spleen, and additionally could significantly improve interleukin-2 （IL-2） and interleukin-6 （IL-6） secretion of blood and spleen lymphocytes. These effects were not observed in mice fed a 2 amino acids mix （glycine and glutamine）. These evidences indicated that an efficient digestion of Gpp polymer protein could be achieved when ingested into the animal gut. The expression system in this study provides a potential production method for not only Gly-Gln dipeptide but also other short bioactive peptides.