中文摘要：

【目的】脂肪酸转位酶（fatty acid translocase,FAT/CD36）是介导脂肪酸跨膜转运和脂肪细胞聚脂的重要载体蛋白。本试验采用主动免疫法研究FAT/CD36在鸡脂肪沉积调控中的作用。【方法】将FAT/CD36膜外区抗原表位基因片段克隆入表达载体pET-32a（＋）,并转化在大肠杆菌BL21（DE3）,构建FAT/CD36融合蛋白表达载体。主动免疫试验选取22日龄黄羽肉鸡60只,按公、母各随机分为2组,共4组。公鸡和母鸡的试验组分别在第34、49、和63天肌肉注射1mg重组鸡FAT/CD36融合蛋白,以牛血清白蛋白（bovineserumalbumin,BSA）为对照。【结果】重组菌表达分子量约为29kD的鸡FAT/CD36融合蛋白,在0.1mmol·L-1IPTG诱导6h后,目的蛋白表达量占菌体总蛋白的32%。表达产物主要以包涵体的形式存在,经纯化并透析复性后得到高纯度的FAT/CD36融合蛋白。主动免疫后,公鸡和母鸡试验组的血清抗FAT/CD36抗体水平逐渐升高,并在首次免疫后显著高于各自对照组。主动免疫FAT/CD36能特异性降低公鸡的腹脂率,但对母鸡无显著性影响。试验组与对照组皮下脂肪厚度差异不显著。【结论】FAT/CD36对鸡脂肪调控具有典型的性别特异性和部位差异。试验结果为进一步阐明禽类脂肪组织特异性沉积的分子机制提供理论依据。

英文摘要：

[Objective] Fatty Acid Translocase （FAT/CD36） is an important carrier protein involved in the transmembrane transportation of fatty acids and cellular triglyceride accumulation. This experiment was designed to get a better understanding of the role of FAT/CD36 in the adipose deposition by active immunization method. [Method] Chicken FAT/CD36 extracellular antigen domain partial gene fragment was cloned, inserted into the prokaryotic expression vector pET-32a（＋） between BaraH Ⅰ and Xho Ⅰ sites, and translated in Escherichia coli BL21 （DE3）. Then, sixty 22-day-old yellow-feathered broilers were randomly divided into male, female control groups and two experiment groups inoculated i.m. with 1 mg of the chicken FAT/CD36 and bovine serum albumin （BSA） immunogens on 34 d, 49 d and 63 d, respectively. At the end of trial, all birds were sacrificed for the fat deposition evaluation. [Result] A 29 kD recombinant chicken FAT/CD36 fusion protein was expressed in Escherichia coli BL21 （DE3）. After induced for 6 hours by adding IPTG to a final concentration of 0.1 mmol·L^-1, the fusion protein amounted up to 32% of the total bacterial protein expressed and existed mainly in inclusion body, which was further purified by affinity chromatography and dialysis. The levels of serum anti-FAT/CD36 antibody in both male and female treatment groups gradually elevated and were significantly higher than that in each control group after the first immunization. It was interesting to find that the active immunization of FAT/CD36 specifically decreased the percentage of visceral fat in male but not female broilers, whereas the thickness of subcutaneous fat was unchanged. [Conclusion] The results indicated the role of chicken FAT/CD36 in the regulation of adipose with gender- and site-specific manner, which provided a further understanding of the fat deposition mechanism in avian.