中文摘要：

本文建立了高效液相色谱-电喷雾飞行时间质谱（HPLC—ESI-TOF／MS）联用技术用于海洋天然药物柄海鞘多种化学成分快速鉴定及其特征图谱研究的方法。对柄海鞘药材进行超声波辅助提取，采用HPLC—ESI—TOF／MS对柄海鞘水提物中多种化合物进行分析，鉴定出柄海鞘水提物中的10种化合物；在化合物鉴定的基础上，建立了柄海鞘药材的HPLC特征图谱，并发现不同批次柄海鞘的质量存在差异。结果表明，本方法可用于柄海鞘药材有效成分的快速鉴定及质量控制。

英文摘要：

A new method based on high performance liquid chromatography-electrospray ionization time of flight-mass spectrometry （HPLC-ESI-TOF/MS） was developed for the rapid identification of active compounds in Styela clava and the development of its specific chromatograms. Samples were extracted by ultrasonic-assisted extraction, and the extraction conditions were optimized. The developed HPLC-ESI-TOF/MS method was used to identify the components in Styela clava extract, and a specific chromatogram based on HPLC analysis was established. Ten compounds in Styela clava extract have been primary identified by HPLC-ESI-TOF/MS on-line detection combined with literature review. The result of similarity evaluation for specific chromatograms indicated that the quality of different Styela clava samples was not entirely consistent. This method has the advantages of simple operation, rapid measurement and it is a powerful tool for identification of active components in Styela clava and its quality control.