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中文摘要：
疱疹病毒（HPVs）庞大而复杂的基因组以及使用常规的在真核细胞中的重组操作技术一直使得HPVs的遗传分析颇具挑战性.近几年发展起来的以细菌人工染色体（BAC）为基础的HPVs全长感染性克隆是全新的技术,它允许HPVs基因组以BAC质粒的形式在大肠杆菌中保存、增殖和遗传修饰.与在真核细胞中相比,在大肠杆菌中操作的重组技术具有更快速、安全而有效的优点.BAC质粒转染真核细胞可重建子代病毒,从而促进了在整个基因组中对HPVs基因功能的研究.以EB病毒为例,介绍了基于BAC的HPVs基因组感染性克隆技术的原理、建立和突变方法,并讨论了该技术的应用前景.
英文摘要：
The genetic analysis of herpesviruses has been a constant challenge, due to the large, complex genomes of herpesviruses and mutagenesis of viral genes by conventional recombination methods in cell cuhure. Recently, a completely new approach for full-length infectious clones of herpesviruses based on bacterial artificial chromosomes （BACs） has been developed. This technique allows the maintenance, propagation and genetic modification of the viral genome as a BAC plasmid in E. coli, thus making the procedures fast, safe and effective in prokaryotic cells. This technique also makes it possible for the reconstitution of viral transfection of the BAC plasmid into eukaryotic cells, thereby facilitating the analysis of the context of genome. In this presentation, Epstein-Barr virus was used as an example progeny or mutants by viral gene functions in to describe the principle, establishment of the technique and mutation introduction into the BAC plasmid, and to discuss the perspective in the use of BAC-cloned herpesviruses.
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