抑制消减杂交(SSH)与基因芯片技术是筛选差异表达基因的新方法,因其快速、高效等特点而得以广泛应用。SSH或基因芯片技术单独使用存在不同程度的缺陷或不足,但若将两种技术结合,则能充分发挥各自优势,并能最大限度弥补其不足,从而成为较为理想的克隆系统。
Suppression subtractive hybridization (SSH) and gene chip are new technologies developed in recent years, which are used to screen differential expression genes. They are widely applied because of their expeditiousness and high efficiency. Similar to other technologies, suppression subtractive hybridization and gene chip have certain limitations respectively. It will be a perfect clone system when they are coupled with each other to exert their advantages and to make up their limitations well.