测定了海南岛南渡江和万泉河2个水系33尾须鲫线粒体控制区5'端520 bp序列,共发现5个变异位点,其中简约信息位点4个,没有插入、缺失,转换颠换比为4,定义了5个单倍型。万泉河和南渡江群体的单倍型多样度分别为0.6762(±0.1006)与0.6993(±0.0435),核苷酸多样度分别为0.00249(±0.001849)与0.00260(±0.001887),二者均处于较低水平。中性检验结果显示Fu's Fs为正值且不显著(0.288,P〉0.1),核苷酸不对称分布不呈明显单峰,表明海南须鲫历史上没有发生快速种群扩张,种群大小相对稳定。2个群体间没有显著遗传分化(Fst=0.03638,P〉0.1),海南须鲫群体可作为一个管理保护单位。
Five hundred and twenty bp nucleotide sequences in the 5' terminal part of the mtDNA D-loop control region of 33 Carassboides cantonensis from 2 populations(Nandujiang and Wanquanhe) of Hainan island were determined.5 mutation sites defined 5 haplotypes, of which 4 sites were parsimony-informatic, and no insertions and deletion was found, transition transversion ratio was 4. The haplotype diversity of Wanquanhe and NanduJiang group were 0.6762(±0.1006) and 0.6993(±0.0435), respectively, and the nucleotide diversity were 0.00249(±0.001849) and 0.00260(±0.001887),respectively, both at a relative low level. Neutral tests showed that the Fu's Fs wasn't significant negative(Fu's Fs=0.288,P0.1), and mismatch analysis wasn't obvious unimodal, which suggested that Carassioides cantonensis in Hainan island might not have experienced rapid population expansion and they were demographically stable in the history. No significant genetic differentiation was found between the 2 groups(Fst =0.03638, P 0.1), so they could be maintained as a single management protection unit.