中文摘要：

目的研究海风藤提取物对β淀粉样蛋白（Aβ寡聚体诱导小胶质细胞激活的影响。方法模拟体内生理条件诱导Ap单体形成Ap寡聚体，原代培养新生大鼠小胶质细胞，分别用Ap25—35寡聚体、A1325—35寡聚体＋海风藤提取物、A1325—35寡聚体＋DMSO干预小胶质细胞，并设空白对照组，48h后测上清液中白细胞介素1β（IL-1β）和白细胞介素6（IL-6）水平，并比较各组间炎性因子差异。结果Ap寡聚体组上清液中IL-1β、IL-6水平明显高于空白对照组[IL-1β：（67．06±1.79）pg／mL vs．（36．30土1．40）pg／mL；IL6：（181．14±4．46）pg／mL73S．（110．90±4．62）pg／mL，均P〈0．05；；Aβ寡聚体组上清液中IL-1β、IL-6水平明显高于Ap寡聚体＋海风藤提取物组（IL-1β：（63．24±2．00）pg／mL，IL-6：（170．34±3．47）pg／mL，P〈0．053；Aβ寡聚体组上清液中IL-1β、IL-6水平和A8寡聚体＋DMSO组[IL-1β（68．26±1．38）pg／mL，IL-6（184．7±6．06）pg／mL]比较无统计学差异。结论Ap寡聚体能激活小胶质细胞；海风藤提取物能明显抑制Ap寡聚体诱导小胶质细胞的激活。

英文摘要：

Objective from mieroglia induced by Aβ without Piper kadsura extract. the Aβ25-35 oligomers group, DMSO group. 48 hours later, inflammatory factors between （36.30±1.40） pg/mL, IL-6： [ （181.14±4.46） pg/mL] in To explore the effect of Piper kadsura extract on inflammatory factor secretion oligomer. Methods Primary microglia was activated with Aβ oligomer with or The cultured microglial cells were divided into 4 groups： the blank control group, the Aβ25-35 oligomers± Piper kadsura extract group and the Aβ25-35 oligomers IL-I and IL-6 in the supernatant were measured by ELISA kit. The difference of each group was compared. Results Compared with the control group [IL-10 （110.9±4.62） pg/mL], the level of IL-1β [ （67.06±1.79） pg/mL] and IL 6 the supernatant of Aβ oligomer group increased significantly （P〈0.05） ; the level of IL-1β and IL-6 in the supernatant of Aβ oligomer group was significantly higher than that in the Aβ oligomer ± Piper kadsura extract group [-IL-1] （63.24±2.00） pg/mL, IL-6： （170.34±3.47） pg/mL, P〈0.05]. There was no difference between the level of IL-1β and IL-6 in the supernatant of Aβ oligomer group and Aβ oligomer DMSO group [IL-1β （68.26±1.38） pg/mL, IL-6： （184.7±6.06） pg/mL]. Conclusions Aβ oligomer can activate microglia Piper kadsura extract can significantly inhibite the A oligomer induced microglial activation.