中文摘要：

目的探讨Aβ寡聚体（Aβo）激活小胶质细胞释放相关炎性因子的作用及海风藤提取物对该过程的影响。方法取Wistar乳鼠的大脑皮层,进行小胶质细胞原代培养、分离、纯化并鉴定;采用醇提取法制备海风藤提取物,将提取物与DMSO按4∶1混合后加生理盐水并用0.22μm微孔滤膜过滤;将纯化后的小胶质细胞按相同密度种植于48孔板,分为空白对照组、Aβo组、Aβo＋海风藤提取物组和Aβo＋DMSO组;采用酶联免疫吸附法（ELISA）分别测定培养基上清液中肿瘤坏死因子-α（TNF-α）、转化生长因子-β1（TGF-β1）的浓度。结果与空白组对照相比,经Aβo处理的小胶质细胞产生的炎性因子明显增加;Aβo＋海风藤提取物组的炎性因子浓度低于Aβo组（P〈0.05）。结论 Aβo能激活小胶质细胞并导致其释放大量炎性因子,而海风藤醇提取物能抑制该过程中炎性因子的释放。

英文摘要：

Objective To explore the effects of oligomeric fornas of β-amyloid protein （Aβo） on microglia and to analyze the influence of Piper Kadsura Ohwi extracts on this progress. Methods Microglia taken from newborn Wistar rats （ within 3days） was cultured, purified and identified. Piper Kadsura Ohwi was extracted by using ethanol. Such an extract was blended with DMSO in the proportion of 4：1 followed by being filtered and concentrated, then normal saline solution （NS） was added and the mix was filtrated by microporous filtering film. Microglia inoculated into 96-well plates was divided into 4 groups ： blank group, Aβo group, Aβo ＋ KPSE group and Aβo ＋ DMSO group, and cultured for 48 hours. TNF-a and TGF-β1 levels in cell supernatant liquid were detected by enzyme-linked immunosorbent assay （ELISA）. Resuits The expression of TNF-a and TGF-131 was increased by the stimulation of Aβo compared with the blank group. The expression of inflammatory factors in KPSE group was lower than the control group. Conclusion Micoglia could be activated and the level of inflammatory factors could be increased by Aβo , and these incidents could be suppressed by KPSE.