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中文摘要：
目的原核表达猪链球菌2型甲基受体趋化蛋白编码基因05SSU0273。方法通过PCR方法检测该基因在不同血清型猪链球菌中的分布情况，然后利用获得的猪链球菌2型05SSU0273基因片段，构建重组表达载体pET32a：：05SSU0273。将质粒转入E．coliBL21中，经IPTG诱导，SDS-PAGE和westernblot实验证实目的基因的表达，然后以His亲和层析柱纯化重组蛋白。结果在S．suis235个血清型标准株中，有20株分离株扩增出目的条带。重组质粒可在宿主菌中高效表达，经镍柱亲和层析得到相对分子质量为43kD的重组蛋白。结论本实验成功获得S．suis2MCP蛋白，为研究其在猪链球菌2型致病过程中发挥的作用奠定了基础。
英文摘要：
To study the biological function of the methyl-accepting chemotaxis protein （MCP） gene 05SSU0273 and its products in Streptococcus suis serotype 2, a pair of specific primers was designed for the gene 05SSU0273, and the target DNA fragment was successfully amplified using the genomic template of Chinese strains 05ZYH33. Subsequently, target gene was inserted into pMD18-T vector, and then subcloned into prokaryotic expression vector pET32a, generating a recombinant ex- pression plasmid pET32a： ： 05SSU0273. Direct DNA sequencing method was used to confirm its correctness. The recombinant plasmid was then transformed into E. coli BL21. After IPTG induction, this bacteria could produce the target MCP protein with 6 ）〈 his-tag which could be purified by affinity chromatography system. The results of SDS-PAGE and Western-blot showed that the fusion protein had a relative molecular weight of 43 kD. Moreover, MCP gene had been detected in 20 of 35 S. suis strains with different serotypes. This prevalence indicated that protein MCP might play a role in the survival of S. suis 2 in different hosts. Together, our work laid foundation for functional research on MCP in S. suis 2.
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