中文摘要：

通过构建肌肉组织cDNA文库分离到鳜肌球蛋白轻链2基因（MLC2），基因登录号为FJ428249．MLC2基因cDNA序列全长1206bp，编码区长度为579bp．MLC2基因开放阅读框编码170个氨基酸，具有EF-手相家族蛋白全部4个EF-手相结构．与已报道的其他动物MLC2相比较，所推导氨基酸序列同源性在66．3％～97．6％，其中与Ca^2＋结合区域非常保守，7种鱼氨基酸序列同源性为100％．采用实时荧光定量PCR方法对鳜MLC2发育表达分析表明，MLC2在原肠期开始有低量表达，与原肠期相比，尾芽期、肌肉效应期和仔鱼阶段MLC2表达量随发育阶段渐进而升高．

英文摘要：

The myosin light chain 2（MLC2） gene was isolated from the muscle tissue cDNA library of Siniperca chuasti. The length of the MLC2 cDNA is 1 206 bp, and the open reading frame is 579 bp. The MLC2 encodes 170 amino acids, which includes four EF-hand structures. Compared with the MLC2 of other animals reported, the homology of the deduced amino acids sequences was between 66.3% -97.6%. The primary structures of the Ca^2＋ - binding domain were well conserved among the MLC2s of seven other fish species. The ontogenetic expression analysis by real-time PCR showed that the MLC2 mRNA was first detected in gastrula stage and its expression gradually increased from the tail bud stage to larval stage.