中文摘要：

研究功能性寡糖组合对锦江黄牛的瘤胃固相微生物多样性的影响。选择3头健康且体重相近的安装有永久性瘤胃瘘管的锦江黄牛,采用自身对照方法,试验分3期进行,第1期：对照期,饲喂基础日粮,不添加任何寡糖;第2期：GT1添加期,饲喂基础日粮＋0.8%甘露寡糖＋1.0%果寡糖＋0.8%大豆寡糖,第3期：GT2添加期,饲喂基础日粮＋0.8%甘露寡糖＋1.20%果寡糖＋1.0%大豆寡糖,每期持续14 d。利用PCR-DGGE方法进行分析牛瘤胃固相微生物PCR-DGGE图谱条带数及相似性。结果发现：添加功能性寡糖组合后,对锦江黄牛瘤胃固相微生物PCR-DGGE图谱条带数影响不明显。3头牛3个试验期DGGE图谱的相似度较高。对锦江黄牛瘤胃液相微生物多样性影响有待下一步研究。

英文摘要：

This experiment was conducted binations on the band numbers of DGGE-DNA to study the effects of different functional oligosaccharide cofingerprints of rumen solid bacteria in Jinjiang cattle. Three healthy Jinjiang cattle with permanent rumen cannula and weight of （300 ± 20） kg were selected. By self-contrasted method, the test was divided into three periods, the first period： the control period, basal diet was fed without any addtional oligosaccharides, the second period, the basal diet added with GT1 of functional oligo- saccharide combination was fed, the third period, the basal diet added with GT2 of functional oligosaccharide combination was fed, the experiment lasted 14 days for each period. The band number of PCR-DGGE finger- prints and similarity of rumen solid bacteria in Jinjiang cattle were analyzed. The results showed that compared with the control period, the effects of functional oligosaccharide combinations of GT1, GT2 on the band numbers of DGGE-DNA fingerprints of rumen solid bacteria in Jinjiang cattle were not obvious. The similarity degree of the three trial cattle was higher in the three periods. The effects of different functional oligosaccharide combinations on diversity of rumen liquid bacteria in Jinjiang cattle remain to be determined.