植物乳杆菌KLDS1.0391与76株乳酸菌分别共培养后,测定培养物的抑菌活性和活菌数,判断与乳酸菌共培养对植物乳杆菌细菌素合成的影响及细菌素合成与菌体密度的关系。结果表明:植物乳杆菌KLDS1.0706、KLDS4.0315、KLDS4.0351、罗伊氏乳杆菌KLDS1.0736这4株菌与植物乳杆菌KLDS1.0391共培养后,抑菌活性显著增加(P<0.01)。与罗伊氏乳杆菌共培养过程中细菌素的抑菌活性与植物乳杆菌KLDS1.0391的细胞密度呈现明显的正相关性,并且发现只有活的诱导菌与植物乳杆菌KLDS1.0391共培养才能够诱导细菌素的合成。
The aim of this study was to determine the effects of 76 lactic acid bacteria strains on bacteriocin production by L. plantarum and the relationship between bacteriocin production and cell number. Lactobacillus plantarum KLDS1.0391 was co-cultured with one of 76 lactic acid bacteria strains for the determination of antimicrobial activity and live cell populations. Significantly (P 〈 0.01) increased antimicrobial activity in the supernatants was obtained by the co-culture of Lactobacillus plantarum KLDS1.0391 with L. plantarum KLDS1.0706, L. plantarum KLDS4.0315, L. plantarum KLDS4.0351 or L. reuteri KLDS1.0736. There was a significant positive correlation between the cell density of Lactobacillus plantarum KLDS1.0391 and the antimicrobial activity of the supernatant of co-culture with L. reuteri KLDS1.0736. We also found that bacteriocin production was induced only when L. plantarum KLDS1.0391 was co-cultured with living cells of inducing strains.