中文摘要：

通过三因素三水平的Box-Behnken设计,利用响应面法对影响加压CO2杀菌效果的主要因素（压力、温度、处理时间）进行研究,以枯草芽孢杆菌芽孢的致死率为响应值,确定在乳中致死芽孢的最优条件,并辅助透射电镜对芽孢超微结构进行观察.结果表明：在温度63℃,压力8 MPa,处理时间101 min的条件下,枯草芽孢杆菌芽孢的致死率可达86.1％,即可将大部分芽孢杀死,但达不到完全杀灭的程度.透射电镜观察发现芽孢结构的改变可能是导致其死亡的主要原因之一.

英文摘要：

Response surface methodology based on a three-level three-factor Box-Behnken design of experiment was used to determine the optimal condition of Bacillus subtilis spores inactivation in milk under pressurized carbon dioxide. The critical factors selected for the investi- gation were temperature, pressure and processing time, and the inactivation rate of spores as response function, and through transmission elec- tron microscope（TEM） observe the damage of spores. Results indicated that optimum inactivation condition was as follows： temperature was 63 ℃, pressure was 8 MPa, processing time was 101 rain, the inactivation rate of spores reached to 86.1%, though most of spores have been inactivated, this condition could not be reach the level of inactivation completely. The result of TEM showed that the damage of spore struc- ture which was an important reason for the death of spores.