中文摘要：

本研究观察钙蛋白酶抑制剂（ALLN和calpain inhibitorⅣ）对纤连蛋白（FN）诱导的MCF-10A乳腺上皮细胞-间质转化（EMT）的影响。FN作用MCF-10A细胞48 h后,采用划痕修复实验检测MCF-10A细胞的迁移能力;基质胶包被的transwell小室实验检测细胞的侵袭能力;Western blot法检测波形蛋白（vimentin）、E-钙粘着蛋白（E-cadherin）、锌指蛋白（snail）和钙蛋白酶-2（calpain-2）的表达。研究结果显示,FN诱导MCF-10A细胞形态发生改变;显著增加MCF-10A细胞的迁移和侵袭能力;上调calpain-2、vimentin和snail蛋白表达,下调E-cadherin蛋白水平。ALLN和calpain inhibitorⅣ能显著抑制FN诱导的MCF-10A细胞形态变化、迁移和侵袭能力增强、vimentin、snail和calpain-2蛋白表达上调及E-cadherin蛋白表达下调。以上研究结果表明,FN诱导MCF-10A乳腺上皮细胞发生EMT可能与上调calpain-2的表达有关,ALLN和calpain inhibitorⅣ能够抑制FN诱导的上皮间质转化。

英文摘要：

The aim of this research is to investigate the inhibitory effects of calpain inhibitors （ALLN and calpain inhibitor IV） on mammary epithelial-mesenchymal transition （EMT） of MCF-10A cells induced by fibronectin （FN）. After FN treatment of MCF- 10A cells for 48 h, cell migration and invasion were determined by scratch repair assay and matrigel coated transwell assay, respectively. Vimentin, E-cadherin, snail and calpain-2 protein expression were measured by Western blot. The results suggest that FN induced morphological changes in MCF-IOA cells, significantly increased the migration and invasion abilities of MCF-IOA cells, upregulated the expression of calpain-2, vimentin and snail, and downregulated the expression of E-cadherin. All such changes by FN were reversed with ALLN and calpain inhibitor IV. In conclusion, the upregulation of calpain-2 was involved in FN-induced EMT of MCF-10A mammary epithelial cells, which may be inhibited by ALLN and calpain inhibitor IV.