中文摘要：

目的探讨泽泻提取物中提取的萜类衍生物单体V-54对小RNA病毒感染增殖的抑制效应及其生物学机理。方法采用KMB17细胞,分别感染HAV-H、PV-Ⅰ和Cox-B2病毒,经V-54处理后检测病毒滴度,分析PV-Ⅰ和Cox-B2病毒增殖抑制的动力学及V-54对KMB17细胞凋亡的影响。结果V-54可抑制3种病毒在KMB17细胞上增殖,PV-Ⅰ和Cox-B2病毒感染滴度比未处理组明显降低,V-54对两种病毒的抑制作用呈剂量依赖性。作用24h内,V-54可使KMB17细胞凋亡率明显增加,随后逐渐恢复正常。结论V-54可抑制特定的小RNA病毒的感染增殖,其机制可能为V-54分子结合到细胞表面的相关受体,导致病毒感染效率降低。

英文摘要：

Objective To investigate the inhibitory effect of terpene derivative V-54 from Alisma orientalis extract on the replication of picornaviridae viruses as well as mechanism of the effect.Methods KMB17 cells were infected with hepatitis A virus H strain（HAV-H）,poliovirus Sabin Ⅰ（PV-Ⅰ）and coxsackievirus B2 strain（Cox-B2）respectively,then treated with V-54 and determined for virus titers,based on which the dynamics of inhibitory effects on replication of PV-Ⅰ and Cox-B2 as well as influence of V-54 on apoptosis of KMB17 cells were analyzed.Results V-54 inhibited the replication of HAV-H,PV-Ⅰ and Cox-B2 in KMB17 cells.The titers of PV-Ⅰ and Cox-B2 in KMB17 cells treated with V-54 decreased significantly as compared with those untreated.The inhibitory effects of V-54 on replications of PV-Ⅰ and Cox-B2 were dose-dependent.The apoptosis rates of KMB17 cells increased significantly within 24 h after treatment with V-54,then decreased to normal level gradually.Conclusion V-54 inhibited the infection and replication of picornaviridae viruses by a potential mechanism of binding to the relevant receptors on cell surface and resulting in the decrease of virus infection efficacy.