欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
生物样本中的蛋白质复杂程度很高,尚无一种分析方法能够全面分析复杂生物样本中的所有蛋白质。实验证明：重复的质谱分析能增加蛋白质的鉴定数量,但鉴定到的蛋白质冗余度很高,高丰度肽段被反复检出。为了降低鉴定冗余度,提高鉴定效率,对线性离子阱-傅立叶变换离子回旋共振质谱仪的串联质谱采集方法进行了研究,建立了基于母离子排除的串联质谱采集方法。此方法能极大降低二级质谱采集的冗余度,提高蛋白质的鉴定效率。
英文摘要：
Due to the complexity of proteome samples,comprehensive analysis to characterize all proteins was still not possible with present methodologies.It has been shown that replicate runs could increase the number of identified proteins.However,the redundancy of protein identifications was high.High-abundant peptides tended to be analyzed repeatedly in different runs.To reduce the redundancy and improve the efficiency of identification,we studied the MS/MS acquisition method of linear ion trap Fourier transform ion cyclotron resonance-mass spectrometry（LTQ-FT） and an acquisition strategy based on exclusion of precursor ions was developed.It proved that the strategy could extremely reduce the redundancy of MS/MS acquisition and improve the efficiency of protein identifications.
同期刊论文项目
同项目期刊论文
Efficient discovery of abundant post-translational modifications and spectral pairs using peptide ma
Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallo
N-glycosylation proteome of endoplasmic reticulum in mouse liver by ConA affinity chromatography cou
A simple and efficient frit preparation method for one-end tapered-fused silica-packed capillary col
Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysi
Facile Synthesis of Mercaptophenylboronic Acid-Functionalized Core-Shell Structure Fe3O4@C@Au Magnet
Novel Proteomic Strategy Reveal Combined Antitrypsin and Cathepsin D as Biomarkers for Colorectal Ca
Concanavalin A-immobilized magnetic nanoparticles for selective enrichment of glycoproteins and appl
SnO2@Poly(HEMA-co-St-co-VPBA) Core-shell Nanoparticles Designed for Selectively Enriching Glycopepti
N-terminal Cyclization of Peptides in Large-scale Protein Identification Based on Biological Mass Sp
Relationship between Sample Loading Amount and Peptide Identification and Its Effects on Quantitativ
Application of open tubular capillary columns coated with zirconium phosphonate for enrichment of ph
Nanozeolite-driven approach for enrichment of secretory proteins in human hepatocellular carcinoma c
Iterative Non-m/z-sharing Rule for Confident and Sensitive Protein Identification of Non-shotgun Pro
A Rapid Isolation and Identification Method for Blocked N-Terminal Peptides by Isothiocyanate-couple
Systematic characterization of the covalent interactions between (-)-epigallocatechin gallate and pe
Fragmentation and Site-Specific Quantification of Core Fucosylated Glycoprotein by Multiple Reaction
Preparation of polypyrrole- coated magnetic particles for micro solid-phase extraction of phthalates
Hydrazide-functionalized magnetic microspheres for the selective enrichment of digested tryptophan-c
Characterization of glycoprotein digests with hydrophilic interaction chromatography and mass spectr
位置：