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中文摘要：
以石英毛细管作为酶固定化的载体,在毛细管内壁上逐步合成树枝形大分子聚酰胺-胺（PAMAM）,再通过交联剂戊二醛将胰蛋白酶直接键合到该大分子的末端氨基上,并对酶固定化条件进行了优化,制备了多层酶反应器.利用该酶反应器对马心细胞色素C等蛋白质进行了酶切,并对酶切的条件进行了优化.实验结果表明,该固定化酶反应器具有较高的酶切效率、良好的重现性和稳定性,可用于蛋白质组学的研究.
英文摘要：
With the capillary as the carrier of enzyme immobilization,poly（amidoamine） was gradually bon-ded to the inner wall of the capillary,then the trypsin was attached at the amino group of poly（amidoamine） with glutaraldehyde,and finally the multi-layer open capillary immobilized enzyme reactor was prepared after enzyme immobilization conditions had been optimized.Cyt-c was chosen as a model protein for evaluation of the performance of the multi-layer open capillary immobilized enzyme reactor,and its digestion conditions were optimized.The results demonstrate that the multi-layer immobilized enzyme reactor has high efficiency,good reproducibility and stability,and can be further applied in future proteomics study.
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