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中文摘要：
本项目针对蛋白质组研究所面临的生物样本蛋白质组成的高度复杂性和现有分析技术与方法的局限性之间的矛盾，围绕规模化蛋白质组分离与鉴定、蛋白质组相对与绝对定量、低丰度蛋白质的富集和翻译后修饰蛋白质的特异性识别与鉴定等一系列问题开展了系统的研究。发展了多种新型蛋白质分离、在线酶解、高丰度蛋白质去除与低丰度蛋白质富集的新技术、新方法和新材料，并应用于复杂体系蛋白质组的分离鉴定；建立了多种蛋白质组相对和绝对定量研究的新技术、新方法和软件，构建了从差异蛋白质筛选到定量确证的技术体系，并应用于重大疾病的定量蛋白质组研究；建立了系列基于化学修饰、同位素标记技术的糖基化和磷酸化蛋白质、多肽的富集、鉴定和定量新技术、新方法和技术平台，并应用于重大疾病和重要生理功能相关的复杂生物体系的蛋白质组研究。本项目的研究成果为构建中国特色的蛋白质组研究技术体系，解决当前蛋白质组研究面临的技术方法的瓶颈问题，提高我国在国际蛋白质组研究领域的学术地位与影响力发挥了重要作用。
结论摘要：
英文主题词proteomics; nano-particle; LC-MS; posttranslational modification; quantitative proteomics
成果综合统计
期刊论文
会议论文
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期刊论文
Efficient discovery of abundant post-translational modifications and spectral pairs using peptide ma
Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallo
N-glycosylation proteome of endoplasmic reticulum in mouse liver by ConA affinity chromatography cou
A simple and efficient frit preparation method for one-end tapered-fused silica-packed capillary col
Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysi
Facile Synthesis of Mercaptophenylboronic Acid-Functionalized Core-Shell Structure Fe3O4@C@Au Magnet
Novel Proteomic Strategy Reveal Combined Antitrypsin and Cathepsin D as Biomarkers for Colorectal Ca
Concanavalin A-immobilized magnetic nanoparticles for selective enrichment of glycoproteins and appl
SnO2@Poly(HEMA-co-St-co-VPBA) Core-shell Nanoparticles Designed for Selectively Enriching Glycopepti
N-terminal Cyclization of Peptides in Large-scale Protein Identification Based on Biological Mass Sp
Relationship between Sample Loading Amount and Peptide Identification and Its Effects on Quantitativ
Application of open tubular capillary columns coated with zirconium phosphonate for enrichment of ph
Nanozeolite-driven approach for enrichment of secretory proteins in human hepatocellular carcinoma c
Iterative Non-m/z-sharing Rule for Confident and Sensitive Protein Identification of Non-shotgun Pro
A Rapid Isolation and Identification Method for Blocked N-Terminal Peptides by Isothiocyanate-couple
Systematic characterization of the covalent interactions between (-)-epigallocatechin gallate and pe
Fragmentation and Site-Specific Quantification of Core Fucosylated Glycoprotein by Multiple Reaction
Preparation of polypyrrole- coated magnetic particles for micro solid-phase extraction of phthalates
Hydrazide-functionalized magnetic microspheres for the selective enrichment of digested tryptophan-c
Characterization of glycoprotein digests with hydrophilic interaction chromatography and mass spectr
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