目的调查河南、江苏和浙江省绵羊的新孢子虫病和弓形虫病的感染状况并进行风险因素分析,为绵羊寄生虫病防控和羊肉肉品安全监测提供基础依据。方法采用间接免疫荧光(IFAT)和改良凝集实验法(MAT)分别检测绵羊血清中的新孢子虫和弓形虫的抗体。统计分析风险因素的OR值及P值。结果绵羊新孢子虫的感染率为11.88%(62/522),弓形虫抗体的阳性率为17.81%(93/522)。地域和流产史是绵羊感染弓形虫的风险因素(P〈0.05),但与新孢子虫的感染不相关(P〉0.05);年龄和性别均与这两种原虫的感染不相关(P〉0.05)。结论河南、江苏和浙江省绵羊新孢子虫病和弓形虫病的阳性率均较高,羊肉肉品安全现状堪忧。该地域绵羊的新孢子虫病流行情况首次报道。
The aim of this research was to investigate the neosporosis and toxoplasmosis for sheep in Henan, Jiangsu and Zhejiang provinces and analyze the risk factors of infeetion, and provide a foundation of prevention and control in this two para- sitosis for sheep and monitoring in mutton safety. The indirect fluorescent antibody test(IFAT) and modified agglutination test (MAT) were used to detect the antibodies of Neospora caninum and Toxoplasma gondii. Odds ratio(OR) and P value were analyzed to distinguish the correlativity of risk factors. The results showed that the seropositive of Neospora caninum was 11.88% (62/522), and the infection rate of toxoplasmosis was 17.81% (93/522). Abortion history and region of sheep were the infection risk factors of toxoplasmosis(P〈0.05), but not for neosporosis(P〉0.05). Meanwhile, gender and age had no relationship of infection in sheep for this two protozoons(P〉0.05). In conclusion, a high positive infection percentage for neosporosis and toxoplasmosis in sheep in Henan, Jiangsu and Zhejiang provinces. Improving and reinforcing the prevention and control measures were urgent, and safety of sheep meat should be focused. This was the first report of the infection of neosporosis in sheep from central and eastern China.