中文摘要：

用亲和色谱研究了两种中药小分子阿魏酸（FA）、丹皮酚（PAE）在人体生理条件缓冲溶液（pH7.4）条件下与人血清白蛋白（HSA）的相互作用.从药物分子在蛋白质分子上有多种类型相互独立的结合位点的假定出发,应用Langmuir吸附模型和竞争置换分析研究了FA,PAE与HSA的竞争性相互作用.结果表明,FA,PAE与HSA之间存在一类位点,且FA与PAE竞争HSA上的indole位点（siteⅡ）.根据热力学参数推测出FA,PAE与HSA之间的作用力主要为氢键作用.从FA,PAE竞争HSA上同一位点的角度,对中医用药中常将含有FA与含有PAE的中药配伍使用,以提高疗效的临床用药现象进行了解释.

英文摘要：

The competition interaction of two active components of Chinese traditional herb,ferulic acid （FA） and paeonol （PAE）,with immobilized human serum albumin （HSA） on chromatographic support under human physiological condition （pH 7.4） has been investigated by high-performance affinity chromatography （HPAC）.Based upon the assumption that there were several independent types of binding sites on an HSA molecule for each drug,the interaction of immobilized HSA with FA and PAE was investigated by frontal analysis and competition displacement analysis.The results showed that there was only a single binding site in the HSA molecule for FA and PAE.As FA and PAE coexisted,they had a direct competition for the indole site （site II） of HSA molecule.According to the thermodynamic parameters,the main force between HSA and FA or PAE was deduced to be hydrogen bonding.The conclusion that both PAE and FA competed with each other for the same binding site of HSA molecule could be used to explain some phenomena in the Chinese medicine clinic,in which the clinical efficacy of each drug can be improved by utilizing the compatibility of Chinese traditional medicine.