中文摘要：

采用亲水作用色谱模式,以二醇基硅胶为固定相,水-有机溶剂为流动相,通过改变流动相中有机溶剂种类及浓度、缓冲盐、有机酸种类及其浓度、柱温等条件,研究了强极性中药组分在亲水作用色谱中的保留行为。结果表明：流动相中水的比例在0～100%（V/V）变化时,溶质保留呈＂U＂型曲线,属于亲水色谱和反相色谱的混合保留机理。水含量在0～50%（V/V）范围时,亲水作用控制溶质的保留。溶质保留随流动相中缓冲盐浓度的增大而增强。对于酸性溶质,其保留随有机酸三氟乙酸、甲酸、乙酸的酸性降低而增大。

英文摘要：

The retention behaviors of six polar compounds from traditional Chinese medicine were investigated with hydrophilic interaction chromatography （ HILIC） by changing kinds of organic solvent and concentration, additives （ salt and organic acid） in mobile phase and column temperature. The retention curves of six solutes exhibit＂U＂shape with the change in the concentration of water from 0 to 100% （ V/V） in mobile phase, indicating a mixed-mode of hydrophilic interaction and reversed phase chromatography. The retention times of all solutes increased with the increase of salt concentration,and the retention of the acidic solutes increased with the decrease of the acidity of organic acid,trifluoroacetic acid,formic acid and acetic acid in the same concentration of the additives in the mobile phase. These results might have some instruction role to select the condition in the separation of traditional Chinese medicine.