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中文摘要：
采用中红外光谱技术对牛奶中掺杂尿素目标物进行检测。配置含有尿素浓度范围为1～18g·L^-1之间18个牛奶样品，分别研究了纯牛奶和掺杂尿素牛奶的红外光谱特性，并进行了对比分析。利用尿素1562cm。处酰胺Ⅲ带C=O伸缩振动吸收峰面积Atl62与I464cm^-1处C—N伸缩振动吸收峰面积A1464的比值A1562／A1464对牛奶中尿素浓度进行线性拟合，研究结果表明A1562／A1464。与牛奶中含有尿素浓度具有一定的线性关系，其相关系数达到0．96以上。该研究对提高乳制品的质量，保护消费者利益具有重要的社会意义和应用前景。
英文摘要：
In the present study, a technique of mid infrared spectroscopy for detection of urea in milk was put forward. Eighteen adulterated milk samples with added different content of urea （1~18 g · L^-1） were prepared. The mid-infrared spectra of these milk samples were measured. The infrared characteristics of pure milk and adulterated milk were studied, and analysis and com- parisons of the differences were carried out. The absorption peak area （A1 s62） of 1 562 cm^-1 , which was assigned to the C--N stretching vibration for urea, and the absorption peak area （A1 464） of 1 464 cm 1, which was assigned to the C=O stretching vibration for amide Ⅲ, were calculated by origin. Linear fitting of relation was made between A1462/A1 464 and urea concentra- tion in milk. The results show that the A1462/A1464 is linear with urea concentration in milk, with a regression coefficient of 0.96. The study is important to improving quality of dairy products and protecting the benefit of consumers, and takes on crucial social significance and application prospect.
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