中文摘要：

目的确定核质桥判定标准，建立^60Coγ射线诱导正常人外周血淋巴细胞中核质桥（NPB）的剂量一效应曲线。方法用^60Coγ射线照射3名健康男性离体外周血，照射剂量分别为0、1、2、3、4、5和6Gy，剂量率为1Gy／min，采用胞质分裂阻滞微核（CBMN）法进行细胞培养、收获、制片、染色。在光学显微镜下分析双核细胞中NPB及微核（MN）。结果在0～6Gy^60Coγ射线照射后，人外周血双核淋巴细胞中的NPB符合泊松分布，且NPB频率随吸收剂量增加而增加（H=19．51，P〈0．01），拟合回归方程为线性平方模式Y=（1．39×10-3）x2＋（4．94×10-3）x（R2=0．981，P〈0．01）。结论成功建立0～6Gy^60Coγ射线诱导正常人外周血淋巴细胞中NPB的剂量一效应曲线。

英文摘要：

Objective To establish the analysis criteria of nucleoplasmic bridges （NPB） and a dose-response curve of NPB frequencies in human peripheral blood lymphocytes irradiated by 60Coy-rays. Methods Human peripheral blood samples were collected from three healthy males, and were irradiated with O, 1, 2, 3, 4, 5 and 6 Gy of 60 Co y-rays. A cytokinesis-block micronucleus assay was carried out to analyze NPBs and micronuclei （MN） in binucleated cells. Results NPBs in binucleated ceils at each dose level of ,/-ray was conformed to the Poisson distribution. The dose-response curve of the y-ray-induced NPB frequencies in human peripheral lymphocytes followed the linear-quadratic model y = （ 1.39 × 10 -3 ）x2 ＋ （4.94× 10-3）x （R2 =0.981, P 〈 0.05）. Conclusions The dose-response curve of NPB frequencies in human peripheral blood lymphocytes induced by 0 -6 Gy 60Co y-rays was established.