中文摘要：

目的：了解使用黄板树根提取液外用熏洗对骨性关节炎（osteoarthritis,OA）患者白细胞介素-18（Interleukin 18,IL-18）在滑液中表达的影响及意义。方法：观察正常膝关节滑膜组织与OA病变滑膜组织在组织形态及病理切片的差异,对OA患者（n=30）使用黄板树根提取液熏洗治疗,分别提取治疗前后的关节滑液,采用酶联免疫吸附测定法（ELISA）定量检测熏洗治疗前后关节滑液中IL-18蛋白的表达水平,并比较其表达的差异。结果：正常滑膜组织肉眼观为淡红色或瓷白色,OA滑膜组织多为粉红色;病理切片显示：相比正常滑膜组织,OA滑膜组织出现明显的血管增生和炎细胞浸润,符合组织慢性炎症病变特征;ELISA检测治疗前OA患者滑液中IL-18的表达含量为（52.625±11.336）pg/ml,治疗后OA患者滑液中IL-18的表达含量为（22.512±2.021）pg/ml,两者之间具备统计学差异（P〈0.05）。结论：黄板树根提取液熏洗治疗可以使OA关节滑液中炎性因子IL-18表达水平下降,提示黄板树根提取液可能对OA有一定的治疗作用。

英文摘要：

Objective： To explore the expressi on and significance of Huangban tree extract for IL-18 in synovial fluid of patients with osteoarthritis（OA）. Methods： Observed the open and pathological difference between normal synovium and OA synovium. Gathered the primary synovial fluid from OA patients（n=30）, and then treated those patients with Huangban tree extract, taken the synovial fluid again after treatment, The concentrations of IL-18 were measured in human OA synovial fluid by enzyme-linked immunosorbent assay（ELISA）, compared the difference. Results： The normal synovium was light red or white porcelain, while the OA synovium was pink. The pathological section of synovium showed： compared to normal synovium, the OA synovium had evident hyperplasy in vessel and infiltrating by inflammatory factors. This was the character of chronic inflammation. The average value of IL-18 in OA before treatment was（52.625±11.336） pg/ml. The average value after treatment was（22.512±2.021） pg/ml. There was a significant statistics difference between them（P〈0.05）. Conclusion： After the treatment of Huangban tree extract IL-18 is lower expressed in synovial fluid of OA patients, it may play an important role in the treatment of OA.