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新疆引进羊驼遗传多样性与系统发育研究
  • ISSN号:1671-7236
  • 期刊名称:《中国畜牧兽医》
  • 时间:0
  • 分类:Q785[生物学—分子生物学]
  • 作者机构:新疆畜牧科学院畜牧业质量标准研究所,乌鲁木齐830000
  • 相关基金:公益性行业(农业)科研专项(201303119-4)
中文摘要:

为充分了解引进羊驼的遗传多样性和系统发育关系,本试验采集了新疆3个地区(阿勒泰市青河县、塔城市和天山野生动物园)39只羊驼血液样品,对其线粒体D-loop基因进行了扩增和测序,得到长度为733bp的片段序列,群体单倍型多样度(Hd)为0.954,核苷酸多样性(Pi)为0.0091,基因流(Nm)为1.97。39条序列共定义了25种单倍型,其中单倍型H7是群体共享最多的单倍型。3个地区羊驼群体之间的遗传距离很小,为0.002~0.003;羊驼与美洲驼、原驼、骆马间的遗传距离为0.002、0.004、0.017,与双峰驼的遗传距离最大,为0.050~0.051,聚类分析发现,3个地区羊驼基本聚为一大支,而且与美洲驼、骆马、原驼亲缘关系较近,双峰驼单独聚为一支。综合分析,新疆引进的羊驼遗传多样性丰富,群体间遗传分化程度较小,基因交流水平较高,羊驼与美洲驼、原驼和骆马之间亲缘关系最近,与双峰驼亲缘关系较远。

英文摘要:

In order to understand the genetic diversity and phylogenetic relationship of the alpacas,39 blood samples of alpaca were collected in 3places of Xinjiang(Qinghe county of Aletai city,Tacheng city and Tianshan wildlife park)and mitochondrial D-loop gene was amplified and sequenced.The results showed that 733 bp fragment was obtained.The population haplotype diversity(Hd),nucleotide diversity(Pi)and gene flow(Nm)was 0.954,0.0091 and 1.97,respectively.25 haplotypes were defined among the 39 gene sequences,and H7 was the most shared haplotype by groups.The genetic distance of three alpaca groups was small(0.002 to 0.003).The genetic distance between alpaca and Lama glama,Lama guanicoe and Vicugna was 0.002,0.004 and 0.017,respectively.The genetic distance between alpaca and Camelus bactrianus was the highest(0.050 to 0.051).The results of cluster analysis showed that three alpaca groups were gathered first,and then gathered with Lamaglama,Lama guanicoe and Vicugna,Camelus bactrianus belonged to another branch along.In conlusion,the genetic diversity and gene flow level of alpacas in Xinjiang was high and the genetic differentiation was relatively small.The genetic relationships between alpaca and Lama glama,Lama guanicoe and Vicugna were close,that with Camelus bactrianus was relatively far.

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期刊信息
  • 《中国畜牧兽医》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院北京畜牧兽医研究所
  • 主编:李琍
  • 地址:中国农业科学院北京畜牧兽医研究所
  • 邮编:100193
  • 邮箱:zgxmsy@caas.cn
  • 电话:010-62816020 62811226 62810371
  • 国际标准刊号:ISSN:1671-7236
  • 国内统一刊号:ISSN:11-4843/S
  • 邮发代号:2-215
  • 获奖情况:
  • 1996年被农业部评为“95中国出版成就展”参展期刊...,1998年在首届全国畜牧兽医优秀科技期刊评比中总分...,2002年被中国期刊协会农业期刊分会和中国农学会评...
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:22619