中文摘要：

旨在探讨miR-193b对黑色素生成关键基因MITF和TYR表达的影响,进而说明miR-193b对黑色素形成的影响。本研究通过细胞转染技术,在绵羊黑色素细胞中过表达miR-193b,检测黑色素细胞中黑色素含量的变化,采用实时荧光定量PCR和Western blot分别对转染细胞中MITF和TYR mRNA和蛋白表达水平进行测定。结果显示：过表达miR-193b的黑色素细胞,黑色素含量明显减少;MITF mRNA和TYR mRNA表达均显著降低（P〈0.01）,分别降低0.233倍和0.198倍;MITF和TYR蛋白表达量也显著降低（P〈0.01）,分别降低0.232倍和0.321倍。本研究表明,过表达miR-193b使黑色素细胞中MITF和TYR表达量降低而间接影响黑色素生成。

英文摘要：

MITF and TYR have been identified to be crucial in the process of melanogenesis,the study aims to investigate the relationship between MITF,TYR and miR-193b,and then to deter- mine the functional roles of miR-193b in the regulation of melanogenesis in melanocyte. The mela- nocytes were transfected with the miR-193b plasmid, tested the assay of melanin, and analyzed ex- pression patterns of MITF and TYR using real-time PCR and Western blotting. The results indi- cated that over-expression of miR-193b in melanocyes reduced the assay of melanin and decreased the expression of MITF and TYR both at the mRNA and protein level,the level of MITF mRNA and TYR mRNA were reduced to 0. 233 times （P〈0.01） and 0. 198 times （P〈0.01） respective- ly,the level of MITF and TYR proteins were reduced to 0. 232 times and 0. 321 times which was significantly different. Results support the role of miR-193b in melanocytes indirectly, which regu- lated the melanogenesis by MITF and TYR.