中文摘要：

采用分子印迹技术与层层自组装技术相结合的方法,利用羧甲基纤维素与溶菌酶制备溶菌酶分子印迹聚合物。首先通过层层自组装的方法将带正电荷的溶菌酶和带负电荷的羧甲基纤维素固定在羧甲基纤维素基材表面,再与三氯化铁反应,采用氯化钠溶液洗脱模板蛋白溶菌酶,制备出溶菌酶分子印迹聚合物。扫描电镜测试结果表明,该溶菌酶印迹聚合物的表面与非印迹聚合物的表面相比变粗糙,并且其表面还出现了一些微孔结构;蛋白质吸附测试结果表明,与非印迹聚合物相比,该溶菌酶印迹聚合物对模板蛋白质的吸附量提高了50%;并且该印迹聚合物对模板蛋白质具有一定的选择性吸附能力;对模板蛋白质的再吸附测试表明,该溶菌酶印迹聚合物具有可重复使用性能。

英文摘要：

A molecularly imprinted polymer （MIP） was prepared by using layer-by-layer （LbL） assembly and molecule imprinting of lysozyme and earboxymethyl cellulose. Firstly, the positively eharged lysozyme and negatively charged car- boxymethyl cellulose were alternately deposited onto the surface of the carboxymethyl cellulose substrate. After reacted with ferric chloride and eluted by 5.0 mol/L sodium chloride solution, the molecularly imprinted polymer of lysozyme was prepared. The surface morphologies of MIP were characterized using scanning electron microscope （SEM）. Compared to non-imprinted polymer （NIP）,the roughness of MIP surface increased. The adsorption capacity and selectivity of MIP were also discussed. The imprinted polymer had 50% higher adsorption capacity toward the template protein than the NIP. The imprinted polymer also had a higher selectivity for the imprinted molecule. In addition, the imprinted polymer showed reusability after several adsorption-desorption cycles.