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中文摘要：
采用微生物转谷氨酰胺酶（mTG）进行修饰,制备了一种生物酶修饰的明胶与纳米银复合生物材料.原子力显微镜测试结果表明纳米银在复合材料中分散比较均匀,没有出现明显的团聚现象.凝胶形成实验和流变性能测试结果表明,mTG酶能够催化明胶/纳米银复合体系进行反应并形成凝胶.与纯明胶材料相比,mTG酶修饰的明胶/纳米银复合生物材料的拉伸强度以及在水体系中的稳定性提高;并且该材料显示出了更好的细菌抑制作用,其对金黄色葡萄球菌的抑制率提高了60.15%.因此,该材料在作为降低细菌感染和炎症反应的皮肤敷料和人工皮肤等生物医用材料领域将具有潜在的应用价值.
英文摘要：
An enzyme-modified gelatin/silver nanoparticle composite biomaterial was prepared in this work. The silver nanoparticles were prepared in aqueous solution at first. Then, the blend of gelatin and silver nanoparticles was used as the composite matrix, and microbial transglutaminase （mTG） was employed as the biological modifier. Atomic force microscopy tests show that silver nanoparticles are distributed uniformly on the surface of the composite material, and no obvious aggregation of silver nanoparticles was observed. Vial inversion tests and rheotogical measurements both confirm that the gel formation and the covalent junctions of the composite can be triggered by microbial transglutaminase. Microbial transglutaminase can crosslink gelatin molecules by catalyzing the transamidation of glutamine and lysine residues to form covalent junctions. The mTG-modified gelatin/silver nanoparticle composite material has better stability in aqueous medium at a moderately high temperature due to the formation of covalent junctions and crosslinks. The results of tensile tests show that the tensile strength of mTG-modified gelatin/silver nanoparticle composite films increases after enzymatic modification. In addition, compared with the mTG-modified gelatin material lacking silver nanoparticles, the mTG-modified gelatin/silver nanoparticle composite material shows better in vitro antibacterial activity against Staphylococcus aureus. The viable cell number of Staphylococcus aureus of mTG- modified gelatin/silver nanoparticle composite material decreases by 60. 15%. With its improved antibacterial activity,stability in aqueous medium, and mechanical strength, this mTG-modified gelatin/silver nanoparticle composite material could be a promising candidate in future applications of skin biomaterial.
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