中文摘要：

首先制备表面带有氨基的聚氨酯基材,再采用层层自组装技术,将两种生物大分子修饰剂（羧甲基纤维素和溶菌酶）层层自组装进行聚氨酯材料的表面修饰。采用原子力显微镜、水接触角测量仪、抗菌活性测试和溶血试验等方法对层层自组装修饰前后的聚氨酯材料的表面性能和生物性能进行了分析。测试结果表明,经过羧甲基纤维素与溶菌酶层层自组装表面修饰后的聚氨酯材料的亲水性提高,未修饰聚氨酯的水接触角为73.0°,而具有5个双分子修饰层的聚氨酯膜片的水接触角为42.3°;与未修饰的聚氨酯相比,层层自组装修饰后的聚氨酯对金黄色葡萄球菌的抗菌活性提高了41.4%;此外,经过层层自组装表面修饰的聚氨酯材料具有更好的血液相容性。

英文摘要：

In this work,amino groups were introduced onto the surface of polyurethane（PU） at first.Then,negatively charged carboxymethyl cellulose and positively charged lysozyme were alternatively deposited onto the surfaces of aminated PU via layer-by-layer（LbL） assembly technique.The surfaces of modified and unmodified PU were investigated by atomic force microscopy and contact angle measurements.The antibacterial activity against Staphylococcus aureus and the hemolysis test of modified and unmodified PU surfaces were tested in vitro,respectively.Then,the surface properties of modified and unmodified PU were evaluated and compared.The results showed that the water contact angle decreased from 73.0° to 42.3° after PU modified by carboxymethyl cellulose and lysozyme through LbL assembly.In comparison with unmodified PU,the antibacterial activity against Staphylococcus aureus of the modified surface decreased 41.4% after assembly of five bilayers.In addition,the hemolysis test indicated that LbL-modified PU surfaces had better blood compatibility than unmodified PU.