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SDF-1α对不同分化状态下NPCs迁移能力的影响
  • ISSN号:0253-9977
  • 期刊名称:细胞生物学杂志
  • 时间:0
  • 页码:223-228
  • 语言:中文
  • 分类:TB383[一般工业技术—材料科学与工程]
  • 作者机构:[1]苏州大学现代丝绸国家工程实验室,江苏苏州215123, [2]苏州大学纺织与服装工程学院,江苏苏州215021, [3]苏州大学基础医学与生命科学学院,江苏苏州215123
  • 相关基金:国家自然科学基金资助项目(30671041,30870642); 江苏高校优势学科建设工程资助项目(苏政办发[2011]6号); 苏州市科技计划项目(SG201046)
  • 相关项目:Rac1在恶性神经胶质瘤细胞诱导的神经干细胞定向迁移过程中的作用研究
中文摘要:

用静电纺丝技术制备了再生桑蚕丝素纳米纤维,并用1-(3-二甲基氨基丙基)-3-乙基碳化二亚胺(EDC)和N-羟基丁二酰亚胺(NHS)进行交联改性,考察了交联改性前后,桑蚕丝素纳米纤维微观形貌及聚集态结构等的变化,采用扫描电镜(SEM)、X射线衍射(XRD)及红外光谱(FT-IR)等测试方法对纳米纤维进行表征。研究结果表明,经EDC/NHS交联改性后,纤维直径由250 nm增加到320 nm,纤维表面变粗糙,且纤维发生弯曲变形;丝素的结构以Silk II为主,并含有部分无规卷曲或Silk I构象;桑蚕丝纳米纤维的力学性能和亲水性有所提高,且交联改性后的纳米纤维具有良好的细胞相容性。

英文摘要:

Regenerated silk fibroin nanofiber prepared by electrospinning methods has crosslinked with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide/N-hydroxy succinimide(EDC/NHS).The morphology of uncrosslinked and crosslinked silk fibroin nanofiber mats was studied by SEM,and its aggregated structure was characterized by X-ray diffraction and FT-IR analysis et al.The results show that after crosslinking,the average diameter of crosslinked nanofiber increases from 250 nm to 320 nm;the crosslinked silk fibroin nanofiber deforms and its surface becomes rough;the porosity of silk fibroin nanofiber mats decreases.At the same time,the secondary structure of crosslinked silk fibroin nanofiber is mainly in Silk II,containing partial random coil or Silk I conformation.The crosslinked silk fibroin nanofiber has better mechanical properties and hydrophilicity than the uncrosslinked materials.In addition,the crosslinked silk fibroin nanofiber shows good biocompatibility.

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