中文摘要：

目的探讨家蚕丝素纳米纤维的排列走向对星形胶质细胞生长发育的影响。方法取新生大鼠大脑皮层,原代培养获得混合细胞培养体系,并通过反复传代对星形胶质细胞进行纯化。将纯化的星形胶质细胞以2.5×105/ml的密度接种至直径为800nm不同走向（平行、乱序）的丝素纳米纤维上,对细胞的存活、铺展以及迁移进行检测。设立多聚赖氨酸（PLL）包被组作为对照。结果成功地建立了新生SD大鼠大脑皮层原代混合细胞培养体系;传至第4代时,星形胶质细胞得到纯化,GFAP阳性率达到92%以上;接种到材料上之后,细胞和家蚕丝素纳米纤维之间表现出很高的亲和力,细胞的胞体和突起按照纤维的走向排列。家蚕丝素纳米材料上的星形胶质细胞的存活率和铺展面积与PLL对照组之间的差异具有统计学意义（P〈0.05）。平行材料上细胞的突起长度比乱序材料上的要长（P〈0.05）,3种材料（平行、乱序、PLL）上的细胞迁移效率差异具有统计学意义（P〈0.05）,其中平行材料上的细胞迁移效率最高。结论家蚕丝素纳米纤维能支持星形胶质细胞的存活和生长,表现出良好的生物相容性;丝素纳米材料的走向影响了细胞的排布以及迁移。

英文摘要：

Objective To investigate the effect of orientation of silk nanofiber on development of astrocytes. Methods Mixed primary cultures were prepared from the cerebral cortex of neronatal SpragueDawley rats. Astrocytes were purified through the repeated passage. The purified astrocytes were seeded onto the aligned/non-aligned silk nanofibers with diameter of 800 nm,and the effects of substrate topography on the survival,extention and migration of the astrocytes were explored. Result we successfully established the cortex astrocytes culture system; GFAP was identified on p4 astrocytes and the percentage of GFAP positive cells was up to 92% . Bodies and processes of astrocytes strict aligned with the orientation of the nanofibers. In contrast to PLL,cells seeded on aligned and random silk nanofibers demonstrated a statistically reduction in survial and extension. Cells cultured on aligned silknanofiber had a longer process than those on other two substrates. Migration efficiency was apparent on the parallel silk nanofibers,being significantly greater than that on random silk nanofibers or PLL. Conclusion The silk nanofiber can support the adhesion,proliferation and migration of astrocytes and the orientation of the nanofibers effect on the distribution and migration of astrocytes.