中文摘要：

建立比格犬血浆中低浓度盐酸二甲双胍高效液相色谱测定方法，用于研究盐酸二甲双胍在消化道各部位的吸收情况。采用高效液相色谱法，以dikma Diamonsil C18（150mm×4．6mm，5μm）柱为分析柱，乙腈-10．0mmol／L磷酸二氢钠水溶液（含7．5mmol／L十二烷基磺酸钠，pH为5．5）（32：68）为流动相，柱温：30℃，检测波长：235nm，流速：1．0mL／min，内标法检测。结果表明，盐酸二甲双胍与内标分离良好，回归方程Y=0．6868X＋（-0．0222）r=0．9995，线性范围0．05～4．0μg／mL。方法回收率在99．78％～107．90％，批内RSD≤3．85％，批间RSD≤5．63％。

英文摘要：

Our objective is to develop a method to quantify low-concentration metformin hydrochloride in beagle dog plasma using High Performance Liquid Chromatography（HPLC）. HPLC is conducted on a Dikma Diamonsil C18 （150 mm×4.6mm, 5μm） with acetonitrile--10. 0 mmol/L sodium dihydrogen phosphate （7.5 mmol/L SDS, pH5. 5）（32.68） as a mobile phase, column temperature 30℃, detection wavelength 235 nm, and flow rate 1.0 mL/min. The internal standard method is used. The results show that the identified characteristics of metformin hydrochloride and the internal standard are distinct. The regression equation is Y =0. 686 8X ＋（-0. 022 2） r=0. 999 5 and the linear range is 0.05-4.0 μg/L. The recovery rates are within 99.78%-107.90%. The intra-precision RSD is less than 3.85%, and the inter-precision is less than 5.63%. The developed method is sensitive, accurate, rapid and simple, and can be adopted to determine low-concentration metformin hydrochloride in beagle dog plasma and in pharmacokinetic study.