中文摘要：

为建立分辨率高、重复性好的血清样品双向电泳技术，从血清样品的水化、等电聚焦、胶条的平衡、胶条的染色等几个方面对双向电泳操作条件进行了分析。结果表明采用以下的操作过程可以获得分辨率高、重复性好的双向电泳结果：样品水化2h（25℃）；胶条泡涨12～16h（25℃）；17cm胶条的等点聚焦程序采用250V线性1h／1000V线性1h／4000V线性2h／8000V线性3小时／8000V线性10h／500V快速0．5h，11cm的胶条的等点聚焦程序采用250V慢速4h／1000V快速2h／4000V快速1h／8000V快速2．5小时／8000V快速7h／500V快速30min；两次平衡各15～20min；银染条件为固定两小时或过夜，敏化50～60min，定影30～40min，显影10min左右，终止10min。这一研究对利用双向电泳分析血清蛋白具有很好的参考价值。

英文摘要：

To establish the serum sample 2-dimensional gels electrophoresis which has the high resolution and good repetition, some aspects in the 2-dimensional gels electrophoresis such as the serum sample solubilization, the gel isoelectric focusing, the gel equilibration and the gel stain are analyzed, and the high resolution and good repetition results are obtained, which are the serum sample solubilization 2h at 25℃, IPG strip rehydration 12h to 16h at 25℃ ,the gel isoelectric focusing at 250 linear ramp lh/1000v linear ramp 1h/ 4000v linear ramp 2h/8000 v linear ramp 3h/8000v linear ramp 10h/500v rapid 30min for 17cm IPG strip or 250v slow ramp 4h/1000v 2h/4000v rapid ramp lh/8000v linear ramp 2.5h/8000v rapid ramp 7h/500v rapid ramp 30min for llcm IPG strip, the gel equilibration twice at 15min to 20rain each time, the silver staining on fixation 2h or to stay overnight/ sensibilization 50rain to 60min/ fixing 30min to 40min/ visualization 10min termination 10min. It is referred to the serum sample analysis in 2-dimensional gels electrophoresis technology.