采用木瓜蛋白酶水解提取鱼鳞胶原蛋白,研究酶解过程中酶解时间、酶解温度、加酶量、pH值对鱼鳞蛋白水解度的影响。通过正交试验设计法,最终确定最佳酶解条件为酶解时间6h、酶解温度50℃、加酶量0.20g、pH5.0。
In this study, the optimal condition for enzymatic hydrolysis of collagen from fish scales were investigated by orthogonal experiments. Papain was used to hydrolyze collagen and effects of four independent variables including hydrolysis temperature, hydrolysis time, enzyme concentration and pH on degree of hydrolysis (DH) of collagen from fish scales were also evaluated. The optimal condition for obtaining the highest hydrolysis yield of collagen from fish scales was 50 ℃ of hydrolysis temperature, 6 h of hydrolysis time, 0.20 g of enzyme amount and pH 5.0.