中文摘要：

目的探讨白细胞介素1α（IL-1α）基因单核苷酸多态性（SNP）与甲型H1N1流感易感性的关系。方法从IL-1α基因启动子区域中选取4个SNP位点rsl800587、rs2856836、rs2856838、rs3783525，针对以上位点建立基于飞行时间质谱分析技术（TOF—MS）鉴定SNP的方法，对167例H1N1流感患者（H1N1组）和192例健康对照人群（对照组）进行检测。确定各SNP位点的等位基因和基因分型；对比两组等位基因和各基因型的频率。结果rsl800587、rs2856836、rs2856838位点具有T和C两种等位基因，包括Tr、TC、CC3种基因型。rs3783525具有A和T两种等位基因，包括AA、AT和Tl 3种基因型。HINl组和对照组rsl800587位点的等位基因频率差异有统计学意义（X2=12．69，P=0．000，OR=2．424，95％CI=1．472～3．993），rs2856836，rs2856838，rs3783525等位基因频率在H1N1组和对照组中差异则无统计学意义。H1N1组rsl800587位点CC纯合子频率低于对照组[72．5％（121／167）比87．0％（167／192），P〈0．05]，杂合子（TC）频率高于对照组125．1％（42／167）比12．5％（24／192），P〈0．05]，而两组Tr纯合子频率差异则不具有统计学意义。H1N1组rs2856836位点杂合子（TC）频率高于对照组[25．7％（43／167）比17．2％（33／192），P〈0．05]，TT基因型与CC基因型频率在两组间差异均没有统计学意义。rs2856838位点对照组TT纯合子频率低于H1N1组[4．2％（8／192）比10．8％（18／167），P〈0．05]，其余2种基因型Tc与cc在两组间差异没有统计学意义。H1N1组rs3783525位点TT纯合子频率高于对照组[21．0％（35／167）比13．0％（25／192），P〈0．05]，两组间的另两种基因型TC与cc差异也没有统计学意义。结论IL-1α仅基因多态性位点rsl800587、rs2856836、rs2856838、rs3783525与甲型H1N1流感易感性相关。

英文摘要：

Objective To determine the association between interleukin-1 alpha （IL-lα） gene single nucleotide polymorphism （SNP） and susceptibility to type A H I N l influenza. Methods Four SNPs （rs1800587, rs2856836, rs2856838 and rs3783525） were selected from IL- lα promoter region for subsequent establishment of the methodology for identifying SNP based on time of flight-mass spectroscopy （TOF-MS）, which was employed to genotype patients with H1Nl（n=167） and healthy controls（n=192）. The alleles of respective SNP were genotyped for comparison on their frequency. Results The rs1800587, rs2856836 and rs2856838 SNPs consisted of T and C alleles and the genotypes of TF, TC and CC. Bycontrast, the rs3783525 included A and T alleles that comprised of AA, AT and Tr genotypes. The difference in allele frequency between H 1N 1 groups and healthy controls reached statistical signifieance （Xz= 12.69, P=0.000, OR--2.424, 95%CI： 1.472-3.993）, whilst the between-group difference in allele frequency was not statistically significant for rs2856836, rs2856838 and rs3783525. Patients with H1N1 yielded a lower allele frequency of CC homozygote for rs1800587172.5%（121/167） vs 87.0%（ 167/192）, P〈0.05] yet higher frequency of TC heterozygote[25.1%（42/167） vs 12.5%（24/192）, P〈0.05]. There was no significant difference in the frequency of TT homozygote. A higher frequency of TC heterozygote for rs2856836 [25.7% （43/167） vs 17.2%（33/192）, P〈0.05] was noted in patients with H1N1, in whom the frequency of TT and CC genotypes did not differ statistically. Patients with H1N1 were linked to a higher frequency of TT homozygote for rs2856838 [4.2% （8/192） vs 10.8% （18/167） , P〈0.05] yet did not differ markedly in the genotypes of TC and CC. Similarly, a higher frequeney of TT homozygote for rs3783525 was found in patients with H1N1 [21.0%（35/167） vs 13.0%（25/192）, P〈0.05], who did not evidence a considerable difference in genotypes TC and CC for two miscellaneous alleles. C