中文摘要：

目的:研究芍药甙(PF)对正常大鼠下丘脑腹内侧核(VMH)神经元兴奋性的影响。方法:应用脑片全细胞膜片钳技术记录了35个正常的VMH神经元的自发放电,观察PF对它们的作用。结果:①当脑片用含PF(300μmol/L)的人工脑脊液灌流后,有22个神经元放电频率明显减少,平均放电频率从(7.1±2.2)Hz减少到(3.2±0.7)Hz(P<0.01);在去极化方波刺激下放电数目也明显减少,洗脱后恢复;②PF对VMH的葡萄糖受体神经元(GRN)表现为明显的抑制作用,与其对非葡萄糖受体神经元的作用相比,差异有统计学意义(P<0.01)。结论:PF能够降低VMH GRN的放电频率,调节下丘脑饱中枢神经元的兴奋性,从而影响动物的摄食行为。

英文摘要：

Objective:To investigate the effects of paeoniflorin(PF)on neuronal excitability in the juvenile rat ventromedial hypothalamic nucleus(VMH).Methods:Whole-cell configuration of the patch clamp technique was used to record the spontaneous discharge of 35 normal neurons in the VMH slice be- fore and after the application of PF.Results:The firing rate of 22 out of 35 neurons significantly reduced from(7.1±2.2)Hz to(3.2±0.7)Hz(P<0.01)after bathing 300μmol/L PF.When these cells were in- jected with a depolariz...