中文摘要：

从 Candida tropicalis 和 Candida lipolytica 的原物在优化 electrofusion (乘 5 的电的脉搏力量 6 kV/cm，脉搏持续时间时间 40 s 和脉搏) 下面被熔化然后在与装载能力的更高的铬完成新遗传型的 YEPD 媒介上再生了。目标 fusant RHJ-004 被它的铬抵抗和铬山锹树能力测试为进一步的研究外面屏蔽。适用性的比较学习证明 fusant 关于装载能力的答案 pH，生物资源集中和铬比它的父母种类有更好的性能。特别为对待低集中 Cr (VI )(20 mg/L ) ，上面， 80% 铬在 pH 从水的阶段被扣押 19。原子力量显微镜学(AFM ) 在房间的有约束力的地点上设想铬的分发，建议改变的表面组织并且细胞内部与它的增加的 biosorption 能力 fusant 伙伴组成。铬的快速的 biosorption 过程跟随兰米尔模型很好。

英文摘要：

Protoplasts from Candida tropicalis and Candida lipolytica were fused under an optimized electrofusion （electrical pulse strength 6 kV/cm, pulse duration time 40μs and pulse times 5） and then regenerated on YEPD media for achieving new genotypes with higher chromium loading capacity. A target fusant RHJ-004 was screened out by its chromium resistance and chromium-sorbing capacity tests for further research. The comparative study of applicability shows that the fusant has better performance than its parent strains in respect of solution pH, biomass concentration and chromium loading capacity. Especially for treating low concentration Cr（VI） （〈20 mg/L）, above 80% chromium is sequestered from the aqueous phase at pH 1-9. Atomic force microscopy （AFM） visualizes the distribution of chromium on the binding sites of the cells, suggesting that the altered surface structure and intracellular constitutes of the fusant associate with its increased biosorption capacity. The rapid biosorption processes of chromium foUow the Langmuir model well.