中文摘要：

为了有效修复广东省贵屿镇环境中十溴联苯醚（Pentabromophenyl ether，BDE209）的污染，从该地区的环境样品中筛选出1株在好氧条件下以BDE209为唯一碳源和能源生长的降解菌J一1，根据菌株的形态特征、生理生化特征以及16SrDNA序列分析，鉴定为苏云金芽孢杆菌（Bacillus thuringiensis）。通过考察不同的环境因素对菌株生长及其降解能力的影响，确定该菌适宜的生长条件为：pH=6．0～9．0，温度30℃，溶氧量4．55～7．50mg／L。降解BDE209的最佳条件为：pH：8．0，温度30℃，溶氧量5．94mg／L。投菌量的增加能较大幅度地提高该菌对BDE209的降解效果。不同的共代谢底物对菌株降解BDE209有不同的影响，乳糖和蔗糖能促进其对BDE209的降解。当体系中存在低质量浓度的Cd^2＋时（≤5mg／L），该菌对BDE209的降解效果得到提高。

英文摘要：

The present paper is concerned with a method for isolating a particular strain of bacteria named J - 1 which can help to degrade the local pollution caused by the over-high concentration of BDE209 in Guiyu, a place in Guangdong. Technically known, strain J - 1 can help to promote the utilization of the sole carbon energy source named BDE209 under the aerobic condition, which can be identified as Bacillus thuringiensis according to its morphological, physiological and biochemical features as well as its 16S rDNA analysis results. Experiment results show that the optimal condition to grow such a strain should be： the appropriate pH is 6.0- 9.0, whereas the proper temperature is 30 % with the dissolved oxygen being between 4.55 -7.50 mg/L, respectively. That is to say, if pH to be chosen is 8.0, the optimal temperature should be chosen 30 ℃ and the dissolved oxygen--5.94 mg/L. It is in such a condition, BDE209 can best display its degradation role, with the best degradation rate being at above 50.0%, and the highest rate up to 73.0%. The increase of inoculum dosage proves to help increase the degradation rate noticeably. The degradation rate can be promoted by adding different cometabolic substrates. Our experiments also prove that the degradation of BDE209 by J- 1 tends to be inhibited in varying degrees by using starch, glucose, phenol and benzoic acid as an external carbon source, with starch being the strongest one. However, when the concentration of Cd^2＋ in the shakes remains at ≤5 mg/L, the degradation rate is likely to be improved because J - 1 is highly tolerable for relatively high concentrations of Cd^2＋, and the degradation of BDE209 may not be significantly inhibited even when the concentrations of Cd^2＋ may go up to 10.0 mg/L.